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Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency

2017; American Academy of Arts and Sciences; Volume: 7; Issue: 12 Linguagem: Inglês

10.21769/bioprotoc.2345

2331-8325

Facundo Rodríguez Ayala, Sebastián Cogliati, Carlos Bauman, Cecilia Leñini, Marco Bartolini, Juan Manuel Villalba, Federico Argañaraz, Roberto Grau,

Agriculture Sustainability and Environmental Impact

Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria.This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media.Because of the high concentration of bacteria in gut worm, dilution is necessary before plating onto growth media.Serial dilutions can reduce the concentration of the original intestinal sample to levels low enough for single colonies to be grown on media plates, allowing for the calculation of the initial counts of bacteria in the intestinal sample.