Produção Nacional
Revisado por pares
Does the use of eCG in TAI protocols improves follicular and luteal function of pregnant and non pregnant crossbred dairy cows
2015; Colégio Brasileiro de Patologia Animal (CBPA); Volume: 12; Issue: 3 Linguagem: Inglês
ISSN
1984-3143
AutoresAntônio de Lisboa Ribeiro Filho, Alessandro Bitencourt Nascimento, Aloisio Bitencourt Nascimento, Marcus Vinícius Galvão Loiola, Alexandra Soares Rodrigues, Priscila Assis Ferraz, J. B. R. Silva, Rodrigo Freitas Bittencourt, Alberto Lopes Gusmão, Marcos Chalhoub,
Tópico(s)Reproductive Physiology in Livestock
ResumoThis study aimed to determine the impact of the use of equine corionic gonadotropin (eCG) on follicular diameter (DFOL), luteal (DCL) and concentration of progesterone (P4) in pregnant and non pregnant cows submitted to a synchronization protocol for timed AI (TAI). A total of 126 crossbred dairy cows were subjected to the following protocol: on a random day of the estrous cycle (Day 0) was applied an intravaginal progesterone device (CIDR®, Zoetis, Sao Paulo, Brazil) associated with 2 mg of estradiol benzoate IM (Gonadiol®, Zoetis). At Day 8 the P4 device was removed and were administered 25 mg of dinoprost trometamina (Lutalyse®, Zoetis) and 1 mg of estradiol cypionate (E.C.P.®, Zoetis). At this time, cows were randomly assigned to one of two treatments: eCG (eCG; n = 61) 300IU of eCG IM (NOVORMON®, Zoetis, Sao Paulo, Brazil) was administred and No eCG (n = 65) without treatment. On Day 10, immedially before the TAI, cows had their DFOL meansured by transrectal ultrasonography. On Day 20, the DCL was meansured and blood collected for P4 concentration determination using the chemiluminescence. Pregnancy diagnosis was perfomed 30 days after TAI by ultrasound. Thus, four groups were established: eCG pregnant (n = 25), eCG not pregnant (n = 30), No eCG pregnant (n = 21) and No eCG not pregnant (n = 37). Statistical analysis was performed using SPSS (version 19) program, considering 5% significance. The DFOL were similar among groups (eCG pregnant, eCG non-pregnant, No eCG pregnant and No eCG nonpregnant: 10.1±3.4; 11.2±3.1; 12.1±2.4 and 11.1±2.9mm, respectively). The DCL were respectively; 19.8±3.8; 21.4±4.2; 20.5±3.7 and 19.9±3.8mm, also did not differ. However, the eCG pregnant group showed significant difference (P = 0.0007) for P4 concentration (16.7±11.1 ng/mL) compared with other groups eCG nonpregnant (8.2±6.7 ng/mL), No eCG pregnant (8.2±5.7 ng/mL) and No eCG nonpregnant (8.7±6.7 ng/mL), these last three did not differ significantly. In conclusion, pregnant cows treated with eCG had better luteal function and consequently higher concentration of P4.
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