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Antibacterial, modulatory activity of antibiotics and toxicity from Rhinella jimi (Stevaux, 2002) (Anura: Bufonidae) glandular secretions

2017; Elsevier BV; Volume: 92; Linguagem: Inglês

10.1016/j.biopha.2017.05.098

1950-6007

Débora Lima Sales, Maria Flaviana Bezerra Morais‐Braga, Antônia Thassya Lucas dos Santos, Antônio Júdson Targino Machado, João Antonio de Araújo Filho, Diógenes de Queiroz Dias, Francisco Assis Bezerra da Cunha, Rogério de Aquino Saraiva, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, José Galberto Martins da Costa, Felipe Silva Ferreira, Rômulo Romeu Nóbrega Alves, Waltécio de Oliveira Almeida,

Antimicrobial Peptides and Activities

The increase in microorganisms with resistance to medications has caused a strong preoccupation within the medical and scientific community. Animal toxins studies, such as parotoid glandular secretions from amphibians, possesses a great potential in the development of drugs, such as antimicrobials, as these possess bioactive compounds. It was evaluated Rhinella jimi (Stevaux, 2002) glandular secretions against standard and multi-resistant bacterial strains; the effect of secretions combined with drugs; and determined the toxicity using two biologic in vivo models, and a in vitro model with mice livers. Standard strains were used for the determination of the Minimum Inhibitory Concentration (MIC), while for the modulatory activity of antibiotics, the clinical isolates Escherichia coli 06, Pseudomonas aeruginosa 03 and Staphylococcus aureus 10 were used. Modulatory activity was evaluated by the broth microdilution method with aminoglycosides and β-lactams as target antibiotics. The secretions in association with the antibiotics have a significant reduction in MIC, both the aminoglycosides and β-lactams. The toxicity and cytotoxicity results were lower than the values used in the modulation. R. jimi glandular secretions demonstrated clinically relevant results regarding the modulation of the tested antimicrobials.