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First Report of Fusarium falciforme (FSSC 3 + 4) Causing Root Rot in Lima Bean ( Phaseolus lunatus L.) in Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 11 Linguagem: Inglês
10.1094/pdis-05-17-0657-pdn
ISSN1943-7692
AutoresEnayra Silva Sousa, Maruzanete Pereira de Melo, J. M. Mota, Ediones M. Sousa, José Evando Aguiar Beserra, Kedma da Silva Matos,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 101, No. 11First Report of Fusarium falciforme (FSSC 3 + 4) Causing Root Rot in Lima Bean (Phaseolus lunatus L.) in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium falciforme (FSSC 3 + 4) Causing Root Rot in Lima Bean (Phaseolus lunatus L.) in BrazilE. S. Sousa, M. P. Melo, J. M. Mota, E. M. J. Sousa, J. E. A. Beserra Jr, and K. S. MatosE. S. Sousa, M. P. Melo, J. M. Mota, E. M. J. Sousa, J. E. A. Beserra Jr†Corresponding author. E-mail: E-mail Address: [email protected], and K. S. MatosAffiliationsAuthors and Affiliations E. S. Sousa M. P. Melo J. M. Mota E. M. J. Sousa J. E. A. Beserra Jr † , Department of Plant Science, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil K. S. Matos , Federal University of Roraima, Agricultural Science Center, 69310-000, Boa Vista, Roraima, Brazil. Published Online:28 Aug 2017https://doi.org/10.1094/PDIS-05-17-0657-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Lima bean is an important legume grown by family farmers, especially in northeastern Brazil. In February 2015, lima bean plants showing wilt symptoms were collected in Tanque (private farms) and Teresina (the experimental area at Federal University of Piauí), Piauí state, Brazil. The evaluated areas showed 5% plant mortality. Roots and stems of plants with wilt symptoms were cut into small pieces and surface-sterilized with 70% alcohol and 1% sodium hypochlorite, dried, and plated in potato dextrose agar (PDA). Two isolates grown on PDA and carnation leaf-piece agar (CLA) at 25°C and under a 12-h photoperiod were morphologically characterized. Monosporic cultures were deposited in the Culture Collection of Phytopathogenic Fungi of the Federal Rural University of Pernambuco, Recife, Brazil (CMM 3357 and CMM 3368). On PDA, the colonies were white/yellowish with cream/tan reverse side, after 7 days. On CLA, the microconidia showed 0 to 2 septa produced in false heads at the tips of long monophialides, and were cylindrical or ellipsoid, with apexes rounded, hyaline, 9.0 to 18.6 μm (avg. 13.8) × 2.0 to 8.7 (avg. 5.3) μm (n = 50). Macroconidia were falcate, hyaline, with pointed apexes, with 3 to 4 septa, measuring 17.7 to 32.8 (avg. 25.2) × 3.5 to 6.0 (avg. 4.7) µm (n = 50). Translation elongation factor (EF-1α) and the second largest subunit of RNA polymerase (RPB2) genes were amplified by PCR and sequenced (GenBank accession nos. KY458974 to KY458977). Maximum likelihood analysis was carried out using the sequences of the two genes from CMM 3357 and CMM 3368 isolates and other species from the Fusarium solani species complex (FSSC). Phylogenetic analysis revealed both isolates most closely related with F. falciforme (100% bootstrap). Pathogenicity was confirmed in plants under greenhouse conditions (25 to 32°C). At 10 days after emergence, the plants were pulled out of the pots and had their roots washed, pruned with scissors, and immersed for 5 min in suspension (1 × 106 conidia ml−1) separately for each isolate. Plants were replanted in sterile soil and after 60 days were evaluated. Design was completely randomized, with seven pots with three plants. The experiment was performed twice. All 42 inoculated plants showed symptoms of root rot. Roots showed black coloring and decrease in size with brownish longitudinal grooves in the cervical region. Internal tissue had a color between red and brown. Dwarfing was observed. There were no symptoms in noninoculated plants. The fungus was reisolated from the diseased plants, and was identified as F. falciforme (FSSC 3 + 4) (O'Donnell et al. 2008) based on its morphological characteristics, genetic analysis, and pathogenicity test, fulfilling Koch's postulates. F. falciforme is considered phylogenetically related to F. paranaense, a species belonging to clade 3 of the FSSC, and pathogenic to soybeans in Brazil (Costa et al. 2016). Despite this, the species that causes rot in azuki bean, mung bean, common bean, and soybean belongs to clade 2 of the FSSC (Aoki et al. 2012). To our knowledge, this is the first report of F. falciforme causing root rot in lima bean in Brazil. This fungus has been reported infecting pine plants (Herron et al. 2015). F. falciforme, a clinically important species, may be common in agricultural environments, including associated with soybean (Chitrampalam and Nelson 2016). This report represents a threat to the lima bean culture.References:Aoki, T., et al. 2012. Mycologia 104:1068. https://doi.org/10.3852/11-303 Crossref, ISI, Google ScholarChitrampalam, P., and Nelson, B., Jr. 2016. Antonie van Leeuwenhoek 109:335. https://doi.org/10.1007/s10482-015-0636-7 Crossref, ISI, Google ScholarCosta, S. S., et al. 2016. Fungal Biol. 120:51. https://doi.org/10.1016/j.funbio.2015.09.005 Crossref, ISI, Google ScholarHerron, D. A., et al. 2015. Stud. Mycol. 80:131. https://doi.org/10.1016/j.simyco.2014.12.001 Crossref, ISI, Google ScholarO'Donnell, K., et al. 2008. J. Clin. Microbiol. 46:2477. https://doi.org/10.1128/JCM.02371-07 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 11 November 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 16 Oct 2017Published: 28 Aug 2017First Look: 5 Jul 2017Accepted: 28 Jun 2017 Pages: 1954-1954 Information© 2017 The American Phytopathological SocietyCited byFusarium falciforme and Fusarium suttonianum cause root rot of melon in Brazil23 January 2023 | Plant Pathology, Vol. 31Species Diversity in the Fusarium solani (Neocosmospora) Complex and Their Pathogenicity for Plants and Humans13 February 2023 | Doklady Biological Sciences, Vol. 507, No. 1Novel causative agents of Fusarium solani species complex causing stem and fruit rot in cucurbit in Taiwan11 April 2022 | Journal of Phytopathology, Vol. 84Fusarium and Neocosmospora Species Associated with Rot of Cactaceae and Other Succulent Plants1 April 2022 | Journal of Fungi, Vol. 8, No. 4Neocosmospora falciformisCABI Compendium, Vol. CABI CompendiumIdentification and virulence of Fusarium falciforme and Fusarium brachygibbosum as causal agents of basal rot on onion in Mexico28 April 2021 | Canadian Journal of Plant Pathology, Vol. 43, No. 5Vigna spp. Root-Nodules Harbor Potentially Pathogenic Fungi Controlled By Co-habiting Bacteria27 March 2021 | Current Microbiology, Vol. 78, No. 5Guava decline: updating its etiology from 'Fusarium solani' to Neocosmospora falciformis19 November 2020 | European Journal of Plant Pathology, Vol. 159, No. 2Phylogeny and pathogenicity of soilborne fungi associated with wilt disease complex of tomatillo (Physalis ixocarpa) in northern Sinaloa, Mexico19 June 2020 | European Journal of Plant Pathology, Vol. 157, No. 4First Report of Fusarium falciforme (FSSC 3+4) Causing Root Rot in Weigela florida in ChinaDong-Xia Shen, Zong-Wen Song, Yi-Ming Lu, and Ben Fan20 January 2020 | Plant Disease, Vol. 104, No. 3Genetic Diversity and Vegetative Compatibility of Fusarium solani Species Complex of Strawberry in SpainEduardo De la Lastra, María Villarino, Juan Diego Astacio, Inmaculada Larena, Antonieta De Cal, and Nieves Capote1 November 2019 | Phytopathology®, Vol. 109, No. 12First Report of Root and Stem Rot Disease on Papaya Caused by Fusarium falciforme in IndiaA. 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