In vitro and in vivo antileukemic activity of B43-pokeweed antiviral protein against radiation-resistant human B-cell precursor leukemia cells
1995; Elsevier BV; Volume: 86; Issue: 11 Linguagem: Inglês
10.1182/blood.v86.11.4228.bloodjournal86114228
ISSN1528-0020
AutoresKG Waddick, Damian E. Myers, Roland Günther, LM Chelstrom, M Chandan-Langlie, JD Irvin, Nilgun E. Tumer, Fatih M. Uckun,
Tópico(s)CAR-T cell therapy research
ResumoB-cell precursor (BCP) leukemia is the most common form of childhood cancer and represents one of the most radiation-resistant forms of human malignancy. In this study, we examined the antileukemic efficacy of the B43 (anti-CD19)-pokeweed antiviral protein (B43-PAP) immunotoxin against radiation-resistant BCP leukemia cells. B43-PAP caused apoptosis of radiation-resistant primary BCP leukemia cells, killed greater than 99% of radiation-resistant primary leukemic progenitor cells from BCP leukemia patients, and conferred extended survival to severe combined immunodeficiency (SCID) mice xenografted with radiation- resistant human BCP leukemia. Furthermore, the combination of B43-PAP and total body irradiation (TBI) was more effective than TBI alone in two SCID mouse bone marrow transplantation models of radiation- resistant human BCP leukemia. Thus, B43-PAP may prove useful in the treatment of radiation-resistant BCP leukemia.
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