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Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses

2017; Brazilian Academy of Sciences; Volume: 89; Issue: 3 suppl Linguagem: Inglês

10.1590/0001-3765201720150476

1678-2690

Raimundo da Silva, Danillo Silva, Ivana Veneza, Iracilda Sampaio, Horácio Schneider, Grazielle Gomes,

Environmental DNA in Biodiversity Studies

In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa.