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High Eg5 expression predicts poor prognosis in breast cancer

2017; Impact Journals LLC; Volume: 8; Issue: 37 Linguagem: Inglês

10.18632/oncotarget.19215

1949-2553

Qin Jin, Fang Huang, Xudong Wang, Huijun Zhu, Yun Xian, Jieying Li, Shu Zhang, Qichao Ni,

14-3-3 protein interactions

// Qin Jin 1 , Fang Huang 1 , Xudong Wang 2 , Huijun Zhu 1 , Yun Xian 3 , Jieying Li 1 , Shu Zhang 1 and Qichao Ni 4 1 Department of Pathlogy, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China 2 Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China 3 Health Insurance Office, Nantong University, Nantong 226001, Jiangsu, China 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China Correspondence to: Qichao Ni, email: nqcuser@163.com Keywords: Eg5, BC, prognosis Received: January 25, 2017 Accepted: April 12, 2017 Published: July 10, 2017 ABSTRACT Eg5 is a motor protein belonging to the kinesin-5 family and has been suggested to exert important function in tumors. In this study, we determined the mRNA and protein expression levels of Eg5 in cancerous and non-cancerous breast tissue by quantitative real-time polymerase chain reaction (qRT-PCR) and tissue microarray immunohistochemistry analysis (TMA-IHC) respectively. The results of 20 fresh-frozen BC samples demonstrated that Eg5 mRNA levels were significantly higher in BC tissues compared with corresponding non-cancerous tissue ( p = 0.0009). TMA-IHC analysis in 127 BC tissues revealed that Eg5 expression obviously correlated with clinicopathologial parameters, including tumor grade ( p = 0.004), ER status ( p = 0.030), Ki67 status ( p = 0.005), molecular classification ( p = 0.026), N stage ( p = 0.015), and TNM stage ( p = 0.001). Kaplan-Meier survival curve indicated that high Eg5 expression ( p = 0.012), Ki67 status ( p = 0.014) and TNM stage ( p = 0.026) were independent factors to predict poor prognosis for patients with breast cancer. Our data suggest that Eg5 is not only overexpressed in BC, it may be also served as a potential prognostic marker.