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Silver‐doped 58S bioactive glass as an anti‐ Leishmania agent

2017; Wiley; Volume: 9; Issue: 1 Linguagem: Inglês

10.1111/ijag.12285

2041-1294

Emanuene Galdino Pires, Roberta Ferreti Bonan, Ítalo Martins Rocha, Ingrid Morgana Fernandes Gonçalves, Joelma Rodrigues de Souza, Laura Helena Vega Gonzales, José Valter Joaquim Silva Júnior, Danyel Elias da Cruz Pérez, Paula Cristina Brígido Tavares, Sydnei Magno da Silva, Renata Pereira Alves‐Balvedi, Luíz Ricardo Goulart, Eliton S. Medeiros, Lúcio Roberto Cançado Castellano, Paulo Rogério Ferreti Bonan,

Laser-Ablation Synthesis of Nanoparticles

Abstract Bioactive glasses ( BG ) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver‐doped 58S BG ( BGA g) against Leishmania species was studied. BG , BGA g1, and BGA g2 belonging to the system 58SiO 2 ∙(36‐x) CaO·6P 2 O 5 · xA g 2 O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol‐gel, and characterized by scanning electron ( SEM ) and atomic force ( AFM ) microscopy, thermogravimetric analyses ( TGA ), X‐ray diffraction ( XRD ), Fourier‐transform infrared ( FTIR ), and surface‐enhanced Raman (Raman‐ SERS ) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57 BL /6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGA g particles. SEM and AFM images showed an irregular and network arranged surface. TGA , XRD , FTIR , and RAMAN ‐ SERS analyses confirmed silver inclusion within BG . None of the BG and BGA g presented toxicity. BGA g2 was effective in controlling promastigote forms under 150 and 300 μg/ mL concentrations of both evaluated species. On macrophage invasion assay, BGA g2 presented reduction in metacyclic forms. For 72 hours, BGA g1 (150 μg/ mL ), BGA g1 (300 μg/ mL ), and BGA g2 in all concentrations were effective against intracellular infection. BGA g could be used as an alternative or complimentary agent to current chemotherapy.