Oral administration of pyrophosphate inhibits connective tissue calcification
2017; Springer Nature; Volume: 9; Issue: 11 Linguagem: Inglês
10.15252/emmm.201707532
ISSN1757-4684
AutoresDóra Dedinszki, Flóra Szeri, Eszter Kozák, Viola Pomozi, Natália Tőkési, Tamás Róbert Mezei, Kinga Makk‐Merczel, Emmanuel Letavernier, Ellie Tang, Olivier Le Saux, Tamàs Arànyi, Koen van de Wetering, András Váradi,
Tópico(s)Parathyroid Disorders and Treatments
ResumoReport12 July 2017Open Access Transparent process Oral administration of pyrophosphate inhibits connective tissue calcification Dóra Dedinszki Dóra Dedinszki Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Flóra Szeri Flóra Szeri Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Eszter Kozák Eszter Kozák Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Department of Immunology, ELTE, Budapest, Hungary Search for more papers by this author Viola Pomozi Viola Pomozi Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Search for more papers by this author Natália Tőkési Natália Tőkési Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Tamás Róbert Mezei Tamás Róbert Mezei Department of Mathematics and its Applications, Central European University, Budapest, Hungary Alfréd Rényi Institute of Mathematics, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Kinga Merczel Kinga Merczel Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Emmanuel Letavernier Emmanuel Letavernier Sorbonne Universités, UPMC Univ Paris 06, UMR S 1155, Paris, France INSERM, UMR S 1155, Paris, France Physiology Unit, AP-HP, Hôpital Tenon, Paris, France Search for more papers by this author Ellie Tang Ellie Tang Sorbonne Universités, UPMC Univ Paris 06, UMR S 1155, Paris, France INSERM, UMR S 1155, Paris, France Search for more papers by this author Olivier Le Saux Olivier Le Saux Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Search for more papers by this author Tamás Arányi Tamás Arányi Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary MITOVASC, CNRS UMR 6015, Inserm U1083, University of Angers, Angers, France Search for more papers by this author Koen van de Wetering Koen van de Wetering Division of Molecular Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands Search for more papers by this author András Váradi Corresponding Author András Váradi [email protected] orcid.org/0000-0002-2722-7120 Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Dóra Dedinszki Dóra Dedinszki Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Flóra Szeri Flóra Szeri Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Eszter Kozák Eszter Kozák Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Department of Immunology, ELTE, Budapest, Hungary Search for more papers by this author Viola Pomozi Viola Pomozi Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Search for more papers by this author Natália Tőkési Natália Tőkési Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Tamás Róbert Mezei Tamás Róbert Mezei Department of Mathematics and its Applications, Central European University, Budapest, Hungary Alfréd Rényi Institute of Mathematics, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Kinga Merczel Kinga Merczel Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Emmanuel Letavernier Emmanuel Letavernier Sorbonne Universités, UPMC Univ Paris 06, UMR S 1155, Paris, France INSERM, UMR S 1155, Paris, France Physiology Unit, AP-HP, Hôpital Tenon, Paris, France Search for more papers by this author Ellie Tang Ellie Tang Sorbonne Universités, UPMC Univ Paris 06, UMR S 1155, Paris, France INSERM, UMR S 1155, Paris, France Search for more papers by this author Olivier Le Saux Olivier Le Saux Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Search for more papers by this author Tamás Arányi Tamás Arányi Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary MITOVASC, CNRS UMR 6015, Inserm U1083, University of Angers, Angers, France Search for more papers by this author Koen van de Wetering Koen van de Wetering Division of Molecular Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands Search for more papers by this author András Váradi Corresponding Author András Váradi [email protected] orcid.org/0000-0002-2722-7120 Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary Search for more papers by this author Author Information Dóra Dedinszki1,‡, Flóra Szeri1,‡, Eszter Kozák1,2, Viola Pomozi1,3, Natália Tőkési1, Tamás Róbert Mezei4,5, Kinga Merczel1, Emmanuel Letavernier6,7,8, Ellie Tang6,7, Olivier Le Saux3, Tamás Arányi1,9, Koen Wetering10,11 and András Váradi *,1 1Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary 2Department of Immunology, ELTE, Budapest, Hungary 3Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA 4Department of Mathematics and its Applications, Central European University, Budapest, Hungary 5Alfréd Rényi Institute of Mathematics, Hungarian Academy of Sciences, Budapest, Hungary 6Sorbonne Universités, UPMC Univ Paris 06, UMR S 1155, Paris, France 7INSERM, UMR S 1155, Paris, France 8Physiology Unit, AP-HP, Hôpital Tenon, Paris, France 9MITOVASC, CNRS UMR 6015, Inserm U1083, University of Angers, Angers, France 10Division of Molecular Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands 11Present address: Department of Dermatology and Cutaneous Biology, PXE International Center of Excellence in Research and Clinical Care, Sydney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA ‡These authors contributed equally to this work *Corresponding author. Tel: +36 203 569135; E-mail: [email protected] EMBO Mol Med (2017)9:1463-1470https://doi.org/10.15252/emmm.201707532 PDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Various disorders including pseudoxanthoma elasticum (PXE) and generalized arterial calcification of infancy (GACI), which are caused by inactivating mutations in ABCC6 and ENPP1, respectively, present with extensive tissue calcification due to reduced plasma pyrophosphate (PPi). However, it has always been assumed that the bioavailability of orally administered PPi is negligible. Here, we demonstrate increased PPi concentration in the circulation of humans after oral PPi administration. Furthermore, in mouse models of PXE and GACI, oral PPi provided via drinking water attenuated their ectopic calcification phenotype. Noticeably, provision of drinking water with 0.3 mM PPi to mice heterozygous for inactivating mutations in Enpp1 during pregnancy robustly inhibited ectopic calcification in their Enpp1−/− offspring. Our work shows that orally administered PPi is readily absorbed in humans and mice and inhibits connective tissue calcification in mouse models of PXE and GACI. PPi, which is recognized as safe by the FDA, therefore not only has great potential as an effective and extremely low-cost treatment for these currently intractable genetic disorders, but also in other conditions involving connective tissue calcification. Synopsis At variance with previous assumptions, orally administered pyrophosphate appears in the circulation in humans and in mice and attenuates connective tissue calcification in mouse models of pseudoxanthoma elasticum (PXE) and generalized arterial calcification of infancy (GACI). Elevation of plasma PPi level in individuals who ingested one dose of Na4PPi, equivalent to 23% of the maximal tolerable daily dose is enough to transiently restore plasma PPi levels. Na4PPi provided in the drinking water in millimolar concentrations inhibited calcification of the dermal sheet of the vibrissae in PXE and in the GACI mice. The inhibitory effect of PPi on the calcification of the arteries was as robust as 75–88% in offspring when heterozygous GACI mothers received 0.3 mM PPi exclusively during pregnacy. Orally taken PPi may represent a simple route to achieve therapeutic solution for treating diseases caused by or accompanied with connective tissue (arterial) calcification. Introduction Physiological mineralization is essential for the normal development of vertebrates and restricted to specific sites of the body. In mammals, biominerals predominantly consist of calcium and phosphate, which form hydroxyapatite. Vertebrates have evolved mechanisms permitting crystallization of calcium and phosphate only at specific sites. Pyrophosphate (PPi) is a central factor in the prevention of the precipitation of calcium and phosphate in soft peripheral tissues (for a recent review see: Orriss et al, 2016). The liver is the most important source of circulatory PPi, via a pathway depending on ABCC6-mediated ATP release (Jansen et al, 2013, 2014), though the exact molecular mechanism of ATP release and the actual substrate of ABCC6 is not known. Within the liver vasculature, released ATP is rapidly converted into AMP and PPi by the ectoenzyme ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1; Jansen et al, 2014). Inactivating mutations in the genes encoding enzymes involved in PPi homeostasis result in rare hereditary calcification disorders which include pseudoxanthoma elasticum (PXE, OMIM 264800), generalized arterial calcification of infancy (GACI, OMIM 208000), arterial calcification due to CD73 deficiency (ACDC, OMIM 211800), and Hutchinson–Gilford Progeria Syndrome (HGPS, OMIM 176670). Absence of functional ABCC6 results in PXE, a late onset ectopic calcification disorder, with lesions found in the skin, eyes, and cardiovascular system (Bergen et al, 2000; Le Saux et al, 2000; Ringpfeil et al, 2000). Biallelic mutations in ENPP1 cause GACI, a condition that can become life threatening shortly after birth due to massive calcification of the large- and medium-sized arteries (Rutsch et al, 2003). While in PXE plasma PPi concentration is reduced to 40% of normal (Jansen et al, 2014), GACI patients have virtually no PPi in their blood, which explains the severity of the disease (Rutsch et al, 2003). Other gene products are also involved in soft tissue calcification affecting PPi homeostasis, such as ANK, which mediates the intracellular to extracellular channeling of PPi (Ho et al, 2000), though it does not play a role in maintaining plasma PPi. Because reduced PPi concentrations in the circulation underlie the ectopic calcification disorders PXE and GACI, a logical treatment for these disorders would be PPi supplementation. Due to the necessity to treat patients lifelong, oral administration would be preferred for such a treatment. Phosphatases are abundantly present in the gut (Ferguson et al, 1968); therefore, it has been always claimed that orally administered PPi cannot reach the circulation and therefore is not effective in inhibiting ectopic calcification (Orriss et al, 2016). We have tested this assumption in healthy human individuals and in mouse models reflecting two human hereditary calcification disorders, PXE and GACI. Results Uptake of PPi in humans and mice First, we tested whether orally consumed PPi is absorbed in humans. Healthy human volunteers (fasting) ingested a solution of tetrasodium pyrophosphate (Na4PPi), resulting in a dose of 40, 67, or 98 mg/kg of body weight (43, 72, 110 mM, pH 8.0, respectively). The ingested amounts of Na4PPi correspond to 13–33% of the maximal tolerable daily intake published by the World Health Organization, WHO (http://www.inchem.org/documents/jecfa/jeceval/jec_2259.htm). This resulted in substantially elevated plasma PPi levels (Fig 1A; with individual differences as shown on Fig 1B) when 98 and 67 mg/kg Na4PPi was taken, while the plasma PPi level was increased only moderately in individuals taking 40 mg/kg (Fig 1A). The time needed to get the plasma PPi back to the baseline level was 240 min at dose 67 mg/kg and 360 min at dose 98 mg/kg (Fig 1A). These data indicate a dose- and time-dependent elevation of plasma PPi concentration. From the data presented in Fig 1A, we calculated the following pharmacokinetic parameters: tmax = 36.7 ± 13.2 min, Cmax = 3.9 ± 1.6 μM, t1/2 = 44.7 ± 16.7 min (single exponential decay) when 98 mg Na4PPi per kg body weight was given. Figure 1. Uptake of PPi from drinking water Oral uptake of tetrasodium pyrophosphate in humans. Volunteers ingested tetrasodium pyrophosphate solutions of 43, 72, 110 mM, pH 8.0, resulting in a dose of 40 mg/kg (n = 10) or 67 mg/kg (n = 10) or 98 mg/kg (n = 9), respectively. Plasma PPi levels were determined before (0 min), and 30, 60, 120, 240, and 480 min after ingestion. The insert shows the differences between the basal plasma PPi level (0 min) and that 30, 60, and 120 min after ingestion. Oral uptake of 98 mg/kg tetrasodium pyrophosphate (n = 9) in human indicating individual differences. Uptake from the oral cavity (n = 5), from the stomach (n = 5), and from the intestine (n = 5) of C57/BI6 mice after ligating the esophagus and applied oral delivery of 100 μl 50 mM PPi; or the pylorus followed by stomach delivery of 200 μl 50 mM PPi and then ligation of the esophagus; or injecting 200 μl 50 mM PPi into the intestine after ligation of the pylorus. In each experiment, including control (n = 7), blood was collected after 15 min and PPi concentrations were determined. Time-course of PPi uptake from the stomach of C57/BI6 mice upon gavage delivery of 200 μl of 50 mM PPi, n = 5–11. Dose-dependent PPi uptake from the stomach of C57/BI6 mice upon gavage delivery of 200 μl of PPi of concentration 0 (n = 6), 1 (n = 6), 10 (n = 7), 25 (n = 7), 50 (n = 8), and 100 (n = 6) mM. Blood was collected for PPi assay after 15 min of delivery. Data information: Data were analyzed by two-tailed Mann–Whitney nonparametric test. Results are expressed as mean ± SEM. Download figure Download PowerPoint Next, we investigated whether supplementation via the drinking water increased plasma PPi levels in mice. In preliminary experiments, we found that in half of the animals, plasma PPi did not increase. This is most probably due to "non-synchronized" drinking by the animals. We therefore directly delivered PPi into the oral cavity, stomach, or small intestine (Fig 1C) and placed ligatures downstream and/or upstream of the site of administration to prevent any transfer of PPi. Unexpectedly, PPi was remarkably well absorbed from all sites tested and we followed the uptake of PPi (50 mM, 200 μl) delivered directly to the stomach over time. PPi was rapidly absorbed from the stomach (Fig 1D) and, as expected, its plasma concentrations depended on the dose given (Fig 1E). Oral PPi inhibits ectopic calcification in Abcc6−/− mice Abcc6−/− mice recapitulate human PXE, with calcifying lesions found in skin, eyes, and blood vessels (Gorgels et al, 2005; Klement et al, 2005). A drawback of the Abcc6−/− mouse model is the relatively late onset of the first symptoms, making it less convenient for rapid screening of new treatments. However, cryo-injury applied to the heart results in calcified lesions within 3–6 days (Doehring et al, 2006), a phenomenon fully dependent on the absence of Abcc6 (Brampton et al, 2014). The lesions showed hydroxyapatite crystal nature as determined by transmission electron microscopy (Aherrahrou, 2003). Next, we determined whether orally administered PPi was able attenuating induced cardiac calcification in PXE mice. PPi provided via the drinking water potently inhibited calcification (PPi was provided in the drinking water a day before the cryo-injury and continued for 4 days) as shown by the reduced calcified area (Fig 2A, calcium deposits stained by Alizarin Red). The extent of inhibition was dose-dependent, with maximal inhibition seen at a PPi concentration of 10 mM, though the cardiac lesions in Abcc6−/− mice receiving drinking water with 1mM PPi already contained more than twofold less calcium (Fig 2B). Figure 2. Oral PPi attenuates induced cardiac and spontaneous calcification of vibrissae in Abcc6−/− mice A. Calcification of the heart of Abcc6−/− mice after 4 days of cryo-injury (drinking water, upper image) or (drinking water with 10 mM PPi, lower image). Ca-precipitations are indicated by arrows, and calcium deposits were visualized by Alizarin Red staining. Scale bar = 1 mm. B. PPi was provided in 0 (n = 8), 1 (n = 8) or in 10 (n = 8) mM concentrations via the drinking water to Abcc6−/− mice starting a day before cryo-injury for a total of 4 days. The calcium content of heart tissue was determined by complexometry. C, D. Show typical Alizarin Red-stained sections of an animal of the control group and that of the 10 mM PPi group, respectively. Abcc6−/− mice were kept on 10 mM PPi (in drinking water) starting at an age of 3 weeks (after weaning) until they were 22 weeks old. The control group was drinking tap water. Tissue blocks with the vibrissae were removed, paraffin-embedded, sectioned, and stained with Alizarin Red for calcium deposits, scale bar = 1 mm. E. The extent of calcification, control (n = 9) and 10 mM PPi (n = 9), was quantified by morphometry as described in the 4. Data information: Data were analyzed by two-tailed Mann–Whitney nonparametric test. Results are expressed as mean ± SEM. Download figure Download PowerPoint A hallmark phenotype seen in Abcc6−/− mice is the spontaneous gradual calcification of the connective tissue surrounding the vibrissae (Klement et al, 2005). We found that providing Abcc6−/− mice PPi-containing drinking water (10 mM) after weaning received for 19 weeks greatly reduced the extent of calcification found in the tissue surrounding the vibrissae. These data show that orally administered PPi not only inhibits the calcification seen after the application of cryo-injury, but also potently inhibits the spontaneous calcification seen in Abcc6−/− mice (Fig 2C–E). Oral PPi attenuates calcification in Enpp1−/− (ttw) mice Tiptoe walking (ttw) mice have an inactivating mutation in Enpp1 (Okawa et al, 1998). Due to the complete absence of Enpp1, these mice have extremely low plasma PPi levels and like GACI patients, which develop extensive calcifications in blood vessels and joints shortly after birth (Nitschke & Rutsch, 2012). Just like Abcc6−/− mice, the Enpp1−/− mice develop extensive calcification of the dermal sheet surrounding the vibrissae. In these animals, this phenotype shows up much earlier than in the PXE mice, however. Orally administered PPi during pregnancy and breastfeeding followed by oral PPi treatment of the pups, resulted in reduced calcification of the dermal sheet surrounding the vibrissae in the Enpp1−/− mice (see Fig 3A–C). We found that treatment of heterozygous Enpp1+/− mothers during their pregnancy with PPi was critical to inhibit the ectopic calcification seen in their Enpp1−/− offspring: Treating Enpp1−/− pups only after weaning did not attenuate ectopic calcification (Fig 3A). We detected a robust effect in the extent of calcification inhibition in the hind limb arteries and in the renal arteries. When PPi in as low as 0.3 mM concentration was provided during pregnancy, calcification was reduced to 12% of the levels found in the control group (hind limb arteries) and to 25% (renal arteries), that is, resulted in 75–88% inhibition (Fig 3D–I). Figure 3. Prenatal PPi treatment of the Enpp1−/− mice attenuates calcification of the tissue surrounding the vibrissae and the arteries of the hind limbs and kidneys A. Calcium content of the tissue blocks of the vibrissae. The heterozygous mothers and their offspring were kept on tap water until the pups were 30 days old ("control", n = 11); Group 1: as the control group, but for 9 days on 10 mM PPi solution after weaning at day 21 (n = 8); Group 2: the mothers and the pups were kept on 10 mM PPi during pregnancy, breastfeeding and for 9 days after weaning (n = 7). Group 3: The mothers were kept on 10 mM PPi during pregnancy (n = 9). Group 4: The mothers and the pups were kept on 1 mM PPi during pregnancy, breastfeeding, and for 9 days after weaning (n = 13). Group 5: The mothers were kept on 1 mM PPi during pregnancy (n = 8). B, C. Typical Alizarin Red-stained sections of tissue blocks with the vibrissae of animals of different groups. Scale bar: 1 mm D. Calcification of renal arteries. The Enpp1+/− mothers were kept on tap water (n = 7) or on 0.3 mM PPi (n = 6) during pregnancy. Offspring was kept on tap water for 55 days. E. Calcium content of the hind limb arteries. The heterozygous mothers were kept on tap water (n = 7) or 0.3 mM PPi (n = 6) during pregnancy. Offspring was kept on tap water for 55 days. F, G. Typical kidney sections of a 55-day-old animal of the control group and of a 55-day-old animal from the group of 0.3 mM treatment only during pregnancy. Sections were stained by the Yasue procedure. Scale bar: 200 μm. H, I. Typical Alizarin Red-stained hind limb arteries of a 55-day-old animal of the control group and those of a 55-day-old animal from the group of 0.3 mM treatment only during pregnancy. Scale bar: 1 mm. Data information: Data were analyzed by two-tailed Mann–Whitney nonparametric test. Results are expressed as mean ± SEM. Download figure Download PowerPoint Discussion Contrary to the general assumption, we found that PPi has bioavailability in humans and mice when administered orally (Fig 1). The observed transient elevation found in healthy volunteers (Fig 1A) indicates that in both GACI and in PXE patients, PPi levels may be transiently raised to the physiological level when 67 or 98 mg Na4PPi/kg of body weight is taken. Importantly, it has been shown that inhibition of calcification in uremic rats and in PXE mice can be achieved by daily intraperitoneal injections of PPi triggering transient increase in plasma PPi levels (O'Neill et al, 2011; Pomozi et al, 2017). We determined a t1/2 = 44.7 min what is rather similar to that published in rat (34.1 min; O'Neill et al, 2011). We also demonstrated significant attenuation of calcification in two different well-characterized mouse models of ectopic calcification disorders, PXE and GACI when the animals were treated with pyrophosphate orally. Furthermore, we demonstrated PPi uptake from the oral cavity and stomach of mice allowing a portion of PPi to escape the rapid hydrolytic decomposition presumably occurring in the intestinal tract. The unexpected observation that 0.3 mM PPi administration was highly effective if applied exclusively during pregnancy (see Fig 3) is likely due to microcrystal formation in the control-treated group before birth. These microcrystals might be mostly absent in Enpp1−/− pups from heterozygous mothers receiving PPi during pregnancy and would therefore not be available to accelerate the calcification process after birth. GACI is often already diagnosed prenatally in the third trimester or at birth (Kalal et al, 2012) when the calcification is already present. The results in the Enpp1−/− mice suggest that under these conditions oral PPi may not be effective in stopping the progression of calcification unless it is started earlier. In summary, oral administration may represent a simple route to achieve therapeutic levels of the physiological, non-toxic metabolite PPi in the blood circulation. Our data indicate that oral PPi has potential to treat two currently incurable diseases, PXE and GACI. Importantly, oral PPi might have broader applicability and be useful in other conditions involving ectopic calcification, such as hypercholesterolemia (Hoeg et al, 1994), diabetes (Kreines et al, 1985), chronic renal insufficiency (Moe & Chen, 2004), β-thalassemia (Aessopos et al, 1992), and heterotopic ossification of traumatized muscle (Jackson et al, 2009). The risk of orally administrated PPi to patients is probably negligible as the WHO considers PPi a non-toxic, physiological, metabolite with a high maximal tolerable daily intake value (MTDI) (http://www.inchem.org/documents/jecfa/jeceval/jec_2259.htm). Moreover, it is extensively used as a food additive, and the Code for Federal Regulation by the FDA states: "This substance is generally recognized as safe when used in accordance with good manufacturing practice" (http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?fr=582.6787). It is also worth noting that PPi would represent a low-cost treatment of connective tissue calcification disorders. Materials and Methods Human study approval The human uptake studies were approved by the National Review Board of the Ministry of Health, Hungary (ETT TUKEB). The actual permit based on the above approval has been issued by National Public Health and Medical Officer Service (ÁNTSZ, authorization number: IF-15816-4/2016). Informed consent was obtained from each volunteer prior to the study and experiments conformed to the principles of Declaration of Helsinki what is indicated in the above document. All patient samples were handled in anonymized form also approved by the above document. Animals and animal studies The RCNS, Hungarian Academy of Sciences Institutional Animal Care and Use Committees, approved the animal studies and were conducted according to national guidelines. C57BL/6J mice designated as wild type were derived from mice purchased from The Jackson Laboratories. Abcc6−/− mice were generated on 129/Ola background and backcrossed into a C57BL/6J > 10 times. Ttw (Enpp1−/−) mice (Okawa et al, 1998) were bred heterozygous due to the severe phenotype seen in the null animals. Both male and female, age-matched Abcc6−/−, Enpp1−/−, and wild-type mice were used. For uptake studies, 15-week-old C57/BI6 animals were used. In cryo-injury calcification experiments, 12-week-old Abcc6−/− animals were studied. Calcification of the vibrissae of Abcc6−/− mice was determined in 22-week-old animals. Calcification of the vibrissae of Enpp1−/− mice was determined in 30-day-old animals. Calcification of the hind limb and kidney arteries of Enpp1−/− mice was quantified in 55-day-old animals. All animals were housed in approved animal facilities at the Research Centre for Natural Sciences, Hungarian Academy of Sciences. Mice were kept under routine laboratory conditions with 12-hour light–dark cycle with ad libitum access to water and chow. Cryo-injury was performed as described previously (Brampton et al, 2014). Oral uptake of tetrasodium pyrophosphate in humans Nine or ten healthy volunteers (age 24–69, fasting) ingested a tetrasodium pyrophosphate solution containing 40 mg/kg or 67 mg/kg or 98 mg/kg (43, 72, 110 mM, pH 8.0, respectively). The ingested amounts of tetrasodium pyrophosphate correspond to 13–33% of the maximal tolerable daily intake published by the World Health Organization, WHO (http://www.inchem.org/documents/jecfa/jeceval/jec_2259.htm). The duration of ingestion was less than one minute. Blood samples were collected before ingestion (0 min) and 30, 60, 120, 240, 360, and 480 min after from the vena cubiti. Pyrophosphate and plasma PPi assay Sodium pyrophosphate tetrabasic decahydrate (BioXtra quality) was purchased from Sigma and used in animal experiments. For human absorption studies, tetrasodium pyrophosphate anhydrous, Code 118 was purchased from ICL Food Specialist (St. Louis, Missouri, USA). Determination of PPi concentration in plasma was performed as described (Jansen et al, 2014). Aliquots from the drinking water during the animal studies were checked for the PPi concentration and found to be stable for at least 4 days. PPi-containing drinking water was changed every second day. Ca-measurement Hearts of Abcc6−/− mice and the tissue blocks harboring the vibrissae of Enpp1−/− mice were digested in 0.15 N HCl for 48 h, and the calcium content was determined by complexometry using the Stanbio Calcium LiquiColor kit (Boerme, TX, USA) following the manufacturer's instructions. Calcification of the vibrissae of Abcc6−/− mice was quantified by histochemistry as described (Klement et al, 2005). Tissue blocks with the hair capsules (vibrissae) were removed, paraffin-embedded, sectioned, and stained with Alizarin Red to visualize calcium deposits. The extent of calcification was quantified by morphometry utilizing image analysis software FIJI (Schindelin et al, 2012). The extent of calcification around the vibrissae was quantified by two investigators in a blinded fashion. Determination of calcification of arteries in the hind limb of Enpp1−/− mice was performed by Alizarin Red staining as described in Kauffenstein et al (2014). Individual images of the arteries were combined using Hugin-Panorama photo stitcher (Free Software Foundation, Inc., Boston, MA USA). The resulting images were then processed using ImageMagick (https://www.imagemagick.org). Kidney tissue sections, 4 μm, were stained by the Yasue procedur
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