Produção Nacional
Revisado por pares
Phenolic compounds of Triplaris gardneriana can protect cells against oxidative stress and restore oxidative balance
2017; Elsevier BV; Volume: 93; Linguagem: Inglês
10.1016/j.biopha.2017.07.050
ISSN1950-6007
AutoresThiago Silva de Almeida, José Joaquim Lopes Neto, Nathanna Mateus de Sousa, Igor Parra Pessoa, Leonardo Vieira, Jackeline Lima de Medeiros, Aline Augusti Boligon, Astrid R. M. Hamers, Davi Felipe Farias, Ad Peijnenburg, Ana Fontenele Urano Carvalho,
Tópico(s)Phytochemistry Medicinal Plant Applications
ResumoThis work aimed to add value to an underexploited plant species from Brazil, Triplaris gardneriana. To that, the phenolic compounds profile of its seed ethanolic extract and fractions was examined by HPLC and the antioxidant capacity assessed using chemical assays as well as in vitro cell imaging. Twelve compounds were quantified and classified as either phenolic acids or flavonoids. The fractionation process did not generate fractions with different compositions except for chloroformic fraction, which showed only 6 out of 12 standard compounds used. DPPH assay revealed samples with a concentration-dependent radical scavenging activity, being methanolic fraction the one with the largest activity (SC50 11.45±0.02μg/mL). Lipid peroxidation assessment, in the presence and absence of stress inducer, showed that particularly the ethanol extract (IC50 26.75±0.08μg/mL) and the ethyl acetate fraction (IC50 6.14±0.03μg/mL) could inhibit lipid peroxidation. The ethyl acetate fraction performed best in chelating iron (48% complexation at 1000μg/mL). Cell imaging experiments showed that the ethanolic extract could protect cells against oxidative stress as well as restore the oxidative balance upon stress induction. In conclusion, T. gardneriana seeds showed a promising phenolic compounds profile and antioxidant activity that may be further exploited.
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