Centriole translocation and degeneration during ciliogenesis in Caenorhabditis elegans neurons
2017; Springer Nature; Volume: 36; Issue: 17 Linguagem: Inglês
10.15252/embj.201796883
ISSN1460-2075
AutoresWenjing Li, Peishan Yi, Zhiwen Zhu, Xianliang Zhang, Wei Li, Guangshuo Ou,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoArticle25 July 2017free access Transparent process Centriole translocation and degeneration during ciliogenesis in Caenorhabditis elegans neurons Wenjing Li Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Peishan Yi orcid.org/0000-0003-4710-8089 Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Zhiwen Zhu Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Xianliang Zhang Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Wei Li Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Guangshuo Ou Corresponding Author [email protected] orcid.org/0000-0003-1512-7824 Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Wenjing Li Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Peishan Yi orcid.org/0000-0003-4710-8089 Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Zhiwen Zhu Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Xianliang Zhang Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Wei Li Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Guangshuo Ou Corresponding Author [email protected] orcid.org/0000-0003-1512-7824 Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China Search for more papers by this author Author Information Wenjing Li1, Peishan Yi1, Zhiwen Zhu1, Xianliang Zhang1, Wei Li1 and Guangshuo Ou *,1 1Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China *Corresponding author. Tel: +86 10 62794766; E-mail: [email protected] EMBO J (2017)36:2553-2566https://doi.org/10.15252/embj.201796883 PDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Neuronal cilia that are formed at the dendritic endings of sensory neurons are essential for sensory perception. However, it remains unclear how the centriole-derived basal body is positioned to form a template for cilium formation. Using fluorescence time-lapse microscopy, we show that the centriole translocates from the cell body to the dendrite tip in the Caenorhabditis elegans sensory neurons. The centriolar protein SAS-5 interacts with the dynein light-chain LC8 and conditional mutations of cytoplasmic dynein-1 block centriole translocation and ciliogenesis. The components of the central tube are essential for the biogenesis of centrioles, which later drive ciliogenesis in the dendrite; however, the centriole loses these components at the late stage of centriole translocation and subsequently recruits transition zone and intraflagellar transport proteins. Together, our results provide a comprehensive model of ciliogenesis in sensory neurons and reveal the importance of the dynein-dependent centriole translocation in this process. Synopsis Ciliogenesis in Caenorhabditis elegans sensory neurons requires dynein-1-dependent dendritic translocation of the centriole to position centriole-derived basal body that forms a template for cilium formation. The centriole is transported from neuronal body to the tip of the dendrite before cilium assembly. The centriolar protein SAS-5 interacts with the dynein light chain LC8. Conditional mutations of dynein-1 block centriole translocation and ciliogenesis. Translocated centrioles lose central tube components and subsequently recruit transition zone and intraflagellar transport proteins. Introduction Our perceptions of the natural world originate from our senses of an unending variety of stimuli in the environment. Sensory organs have developed specialized structures to perform this feat. For example, the mammalian olfactory system is optimized for the detection and discrimination of odorant molecules through odorant receptor proteins that are expressed by peripheral olfactory sensory neurons (OSNs; McEwen et al, 2008; Challis et al, 2015). A bipolar OSN possesses an unbranched axon that projects to the olfactory bulb and a dendrite that extends apically and terminates in a dendritic knob at the epithelial surface. A remarkable feature of OSNs is the formation of olfactory cilia that emanate from the dendritic knob and project into the mucus of the nasal cavity (Menco, 1997; Jenkins et al, 2009). These cilia harbor G-protein-coupled receptors and other proteins that are essential for olfactory signal perception and transduction, making them the sites where the detection of a chemical odor is converted into an electrical response (Berbari et al, 2008). Importantly, each dendritic knob contains 10–30 olfactory cilia of up to 50–60 μm in length, which increases the sensory surface of the ciliated epithelium to amplify the ability of odorant detection (Cuschieri & Bannister, 1975a; Mashukova et al, 2006; Oberland & Neuhaus, 2014). Disruption of ciliary structure or function causes human ciliopathies such as anosmia (inability to perceive odors) or dysosmia (altered odor perception), highlighting the significance of olfactory cilia in odor perception (Toller, 1999; Klysik, 2008; Brown & Witman, 2014). Cilia are microtubule-based organelles in which the ninefold symmetric array of doublet axonemal microtubules is extended from the centriole-derived basal body (Ward et al, 1975; Pedersen et al, 2008; Reiter et al, 2012). The assembly of olfactory cilia has unique features, as multiple cilia are present at the dendritic ending of an OSN (Menco, 1980; Challis et al, 2015; Falk et al, 2015). Cells normally use one centriole to build a single cilium. The multiciliated OSNs are thought to multiply their centrioles in the cell body, and then the centrioles undergo long distance dendritic transport to arrive at the dendritic knob, where they permit the elongation of cilia (Dirksen, 1974; Schwarzenbacher et al, 2005). Immunohistochemistry on ciliogenesis in OSNs has outlined the possible cellular events involved, including dendrite outgrowth, centriole amplification and translocation, and cilium formation (Fig EV1A; Hagiwara et al, 2004; Jenkins et al, 2009). However, the dynamic behavior of centriole and the underlying molecular regulation remain largely unexplored due to the complex developmental processes (at approximately E10–E14 in mouse) and the lack of an experimental methodology for live cell microscopy (Cuschieri & Bannister, 1975a,b; Hagiwara et al, 2004; Jenkins et al, 2009). Click here to expand this figure. Figure EV1. Centriole in cilium formation Schematics of the centriole positioning during ciliogenesis in mammalian olfactory neurons. Fluorescence time-lapse images of the dynamic positioning of centrioles marked with SAS-6::GFP (green) and of the plasma membrane and chromosome (red, labeled with mCherry) during cell division and neuritogenesis. mMaple3::SAS-4 localization in PQR neuron. Schematic (left) and images (right) of the ciliated neurons in C. elegans. Red boxes represent the region of images shown in the left. Cell bodies of amphid neurons locate near the pharyngeal bulb, and cilia are positioned at the tip of dendrites that are extended anteriorly. Phasmid cilia are located at the tip of dendrites that were elongated toward the posterior of C. elegans. Yellow asterisks indicate the location of ciliary base. Data information: Scale bars, 5 μm. Download figure Download PowerPoint The nematode Caenorhabditis elegans offers a compelling model system to explore de novo ciliogenesis in the nervous system. All 60 ciliated cells in an adult C. elegans hermaphrodite are sensory neurons (Ward et al, 1975; Ware et al, 1975; Perkins et al, 1986; Inglis et al, 2007; Doroquez et al, 2014). In C. elegans embryos, previous live imaging studies have shown that sensory dendrites in amphids extend from the cell body via retrograde elongation, such that the growing dendrite is anchored at the presumptive nose while the soma translocates toward the posterior (Sulston et al, 1983; Heiman & Shaham, 2009; Schouteden et al, 2015). As a result, the basal bodies in these neurons do not undergo the dendritic translocation but are initially positioned at dendrite tips where sensory cilia emerge (Perkins et al, 1986; Heiman & Shaham, 2009). However, in mammalian OSNs, centrioles were detected in the middle of emerging dendrites and then arrived at the dendrite tips in mature neurons (Ying et al, 2014), which suggests that centriole translocation is an early step before the assembly of olfactory cilia (Cuschieri & Bannister, 1975a). Moreover, cilium formation in the C. elegans PQR sensory neuron may share similarities with ciliogenesis in mammalian OSNs (Figs 1A and EV1A). During the first larval stage, PQR is generated by asymmetric cell divisions of the QL neuroblast; after migration, PQR elongates its dendrite and constructs a sensory cilium at its dendrite ending (Sulston & Horvitz, 1977; Perkins et al, 1986). We have developed fluorescence time-lapse microscopy to document Q neuroblast division, migration, and dendrite outgrowth in live C. elegans larvae (Chai et al, 2012). Figure 1. Centriole migration and degeneration in the dendrite A. Schematics of the centriole positioning during ciliogenesis in Q neuroblast in Caenorhabditis elegans. Centriole translocates to the dendrite tip to initiate cilium assembly. B, C. (B) Representative fluorescence time-lapse images of the dynamic positioning of centrioles (green, labeled with SAS-6::GFP) and the plasma membrane/chromosomes (red, labeled with mCherry) during ciliogenesis. Time 0 refers to when the leading edge starts to extend from the non-motile cell body to form the dendrite, and the centriole migrates along with dendrite elongation. Note that centriole (1) translocates, whereas centriole (2) stays in the soma, and the centriole displacement defined by the position relative to cell body over time is quantified in (C) (Movie EV1). For details of the quantification, see methods and Fig EV2A; more examples are shown in Fig EV2B. D. At the end of centriole migration, the centriole signal gradually decreases (left). Individual fluorescent centrioles were tracked, and intensity was quantified over time (right). More examples are shown in Fig EV2C. E. No fluorescence of SAS-4, SAS-5, or SAS-6 was detected at the base of the cilia in C. elegans sensory neurons in adult worms. CB, ciliary base, indicated with yellow asterisks. Data information: Scale bars, 5 μm. Download figure Download PowerPoint Here, we use the live imaging protocol, for the first time, to define the subcellular events involved in the formation of a sensory cilium of the C. elegans PQR neuron. We document centriole translocation from the cell body toward the dendrite tip when the dendrite elongates. Using biochemical and genetic approaches, we find that centriole translocation depends on cytoplasmic dynein-1 and that the centriolar protein SAS-5 interacts with the dynein light-chain LC8. We further document the sequential accumulation of transition zone proteins and intraflagellar transport (IFT) proteins during the late stage of centriole translocation. Unexpectedly, centriolar proteins are removed upon the accumulation of transition zone proteins. Taken together, our results establish a comprehensive model of the de novo cilium formation in sensory neurons and provide insights into the same process in the mammalian olfactory system. Results Centriole translocation and degeneration in the dendrite To monitor the dynamic behavior of centriole during ciliogenesis in C. elegans neurons, we labeled the components of the centriole core proteins including SAS-4, SAS-5, and SAS-6 with a fluorescent protein (Leidel & Gonczy, 2003; Delattre et al, 2004; Leidel et al, 2005). Using a CRISPR-Cas9-assisted homologous recombination method, we constructed a mMaple3::SAS-4 knock-in (KI) strain. The fluorescence protein mMaple3 marks the tagged protein with green fluorescence (Wang et al, 2014). The KI animal is indistinguishable from the wild-type (WT) nematodes in animal development. The particle bombardment transformation protocol (Praitis et al, 2001) was previously used to generate an integrant low-copy GFP::SAS-5 or SAS-6::GFP strain. As illustrated in the early studies, these markers label centrioles during C. elegans development (Cabral et al, 2013); for example, the GFP fluorescence of SAS-6::GFP was detected as a single spot at the spindle pole during Q cell asymmetric division and split to double spots (Fig EV1B). To visualize the PQR sensory neuron, we expressed mCherry-tagged histone and plasma membrane proteins under the control of the Q neuroblast-specific promoter Pegl-17 (Figs 1B and EV1B; Ou et al, 2010). To define the subcellular localization of the centriole during PQR development in live C. elegans larvae, we performed fluorescence time-lapse microscopy of the developing larvae at 2-min intervals over a period of 2–3 h. Our recordings usually start when the cell body of PQR arrives at the destination, while the leading edge continues to elongate to form the dendrite (Fig 1B). The dendrite grows out first, and the centriole translocation follows dendritic extension (Fig 1B). Time 0 refers to the start point of the leading edge extension from the non-motile cell body. At approximately 15 min, one centriole moved into the growing dendrite, whereas the other remained immobile in the soma (Fig 1B and C, Movie EV1 and more examples in Fig EV2B). Quantification of the time-lapse movies showed that the motile centriole translocated for 67 ± 22 min for a total distance of 5.1 ± 2.7 μm (Mean ± SD; N = 31). These results provide direct evidence in live animals that the centriole undergoes dendritic translocation to build the sensory cilium. Click here to expand this figure. Figure EV2. Centriole translocation and degeneration A. Schematics of the quantification of centriole positioning. B, C. Additional examples of centriole dynamic positioning (B) and decreased centriole signal (C). Download figure Download PowerPoint Intriguingly, we found that the SAS-6::GFP fluorescence of the motile centriole disappeared in the growing dendrite at the late stage of centriole translocation. A gradual decrease in SAS-6::GFP fluorescence always occurred within 8 ± 2 min at 67 ± 22 min after the dendritic growth initiates, at the position of 5.1 ± 2.7 μm away from the cell body (Mean ± SD; N = 6; the representative images are shown in Figs 1D and EV2C), which suggests that the centriolar protein degradation may be programmed and argues against the possibility that they simply move out-of-focus during time-lapse recording. We showed that the SAS-6::GFP fluorescence from the non-motile centriole in the cell body did not change during our recording and that the dendritic growth appeared to be normal (Figs 1B and EV1B), excluding the possibility that the loss of SAS-6::GFP in the dendrite was caused by photo-bleaching or photo-toxicity. Other centriole markers including mMaple3::SAS-4 could not be detected from the motile centriole in the dendrite but retained at the non-motile centriole in the cell body (Fig EV1C). Consistently, all these centriolar proteins disappeared from the base of cilia of other sensory neurons in the C. elegans amphid and phasmid (Fig 1E). Thus, our live imaging data indicate that centriolar proteins are lost from the motile centriole in the growing dendrite and support the previous observation that the C. elegans centriole generally degenerates (Perkins et al, 1986; Dammermann et al, 2009; Williams et al, 2011). Centriolar proteins are essential for ciliogenesis in Caenorhabditis elegans neurons It is well established that the centriole-derived basal body provides a template for nucleating axonemes. However, C. elegans centrioles do not have a cartwheel at their core but rather a central tube (O'Toole et al, 2003; Pelletier et al, 2006); and the early loss of the core centriolar proteins was detected in the developing dendrite (Fig 1D). We sought to rigorously examine the function of the centriolar protein SAS-4, SAS-5, and SAS-6 during ciliogenesis in C. elegans sensory neurons. To bypass the crucial function of centriolar proteins in cell division and embryonic development, we used the somatic CRISPR-Cas9 technique to generate conditional mutants of centriolar proteins in C. elegans L1 larvae (Fig 2A). We devised this technique to study the embryonically essential genes in Q cell division and migration (Shen et al, 2014). Using the same platform, we expressed the Cas9 endonuclease under the control of a heat-shock-inducible promoter to generate conditional mutants of sas-4, sas-5, and sas-6 (Fig 2A and B). After heat-shock induction of Cas9 expression, T7 endonuclease I (T7EI)-based assays demonstrated that DNA fragments from sas-4,-5,-6-sg strains (-sg for the conditional mutant) were digested into two small fragments of the expected sizes with the indels (insertion–deletion) of 9, 7, and 5%, respectively (Fig 2C). The conditional mutant embryos but not WT embryos exhibited embryonic lethality with the penetrance of 81 ± 4% (sas-4-sg), 94 ± 4% (sas-5-sg), and 85 ± 6% (sas-6-sg; Mean ± SD; N = 78–372; Fig 2D), which are consistent with the reported phenotypes of their RNAi animals (Gonczy et al, 2000; Sonnichsen et al, 2005). Thus, we constructed conditional mutations of embryonically essential centriole genes (sas-4, sas-5, and sas-6). Figure 2. Centriolar proteins are essential for ciliogenesis in Caenorhabditis elegans neurons A. Schematic representation of the cartwheel viewed from the proximal end. The nine symmetric cartwheel central hub is composed of SAS-6 (blue), where SAS-5 (red) localizes at the interior of centriole, and appears to function as a linker between SAS-6 and SAS-4 (green). SAS-4 localizes more toward the periphery of the centriole, which associates with microtubules. B. Gene models of centriolar proteins for generating conditional mutations; exons are shown as boxes, and arrows indicate sgRNA sequences corresponding to exons (Table EV1). C. Representative gels showing the T7EI assay results for sas-4-sg, sas-5-sg, and sas-6-sg. PCR products amplified from the genomic DNA of worms expressing Phsp::Cas9 and PU6::sas-4,5,6-sg after heat-shock treatment. Indels are indicated at the bottom. D. Embryonic lethality was determined by quantifying viable embryos after heat-shock treatment. N = 78–372; Mean ± S.D. (error bars). ****P < 0.0001 based on Student's t-test. N.S., not significant. E. Ciliary morphology (amphid, phasmid, and PQR neuron) in conditional mutants as visualized by OSM-6::GFP. The penetrance was quantified at 0 ± 0% for WT (N = 62), 14 ± 7% for sas-4-sg (N = 76, P < 0.05), 23 ± 7% for sas-5-sg (N = 65, P < 0.01), and 18 ± 1% for sas-6-sg (N = 28, P < 0.001). Yellow asterisks indicate the location of ciliary base. Blue arrows mark the distribution of IFT particles along cilia. F. Transition zone morphology in conditional mutants visualized by MKS-5::mMaple3. The penetrance is 0 ± 0%, 12 ± 3%, 27 ± 10%, and 15 ± 1% for WT (N = 46), sas-4-sg (N = 69, P < 0.05), sas-5-sg (N = 98, P < 0.01), and sas-6-sg (N = 106, P < 0.001), respectively. Yellow asterisks indicate the location of ciliary base. G–I. (G, H) Fluorescence time-lapse images of MKS-5::mMaple3 accumulation in sas-5 mutant animals. Quantification is shown in (I), no accumulation (> 150 min), delayed accumulation (120–150 min), and normal (< 120 min) (N = 49) (Movie EV2). Data information: Scale bars, 5 μm. Download figure Download PowerPoint We next examined the ciliary morphology in these conditional mutant animals. Using an OSM-6/IFT52::GFP reporter that marks an IFT-particle subunit moving along the entire cilium, we found that the GFP fluorescence of OSM-6::GFP was visible in the dendrite but was absent in 14 ± 7%, 23 ± 7%, and 18 ± 1% of sas-4-sg, sas-5-sg, and sas-6-sg animals, respectively (Mean ± SD; N = 28–76; the representative images are shown in Figs 2E and EV1D), indicating the lack of IFT in the absence of the centriolar proteins in amphid and phasmid cilia. The similar lack of OSM-6::GFP fluorescence was also observed in cilia of the PQR neuron, with the penetrance of 15 ± 2%, 33 ± 11% and 30 ± 14% of sas-4-sg, sas-5-sg, and sas-6-sg (Mean ± SD; N = 12–20; the representative images are shown in Fig 2E). We further examined the transition zones in these mutant animals. The transition zone is a subdomain of the cilium characterized by Y-shaped fibers connecting the doublet microtubules and ciliary membrane and functioning as a ciliary gate to regulate IFT (Williams et al, 2011). We generated a mMaple3 knock-in animal of MKS-5 (Meckel-Gruber syndrome) protein to visualize the transition zone and mMaple3-tagged MKS-5 localized at the ciliary base in 100% of the WT adult animals. In consistent with the absence of OSM-6::GFP in cilia, no fluorescence of MKS-5::mMaple3 could be detected at the base of cilia, with the penetrance of 12 ± 3%, 27 ± 10%, and 15 ± 1% in amphid and phasmid cilia of the adult sas-4-sg, sas-5-sg, or sas-6-sg animals, respectively (Mean ± SD; N = 46–106; the representative images are shown in Fig 2F). The TZ defects were also detected in PQR neuron as 18 ± 2%, 40 ± 1%, and 17 ± 4% of sas-4-sg, sas-5-sg, or sas-6-sg animals were lack of MKS-5::mMaple3 fluorescence intensity around their transition zones (Mean ± SD; N = 17–67; the representative images are shown in Fig 2F). We next examined the MKS-5 accumulation in L1 larvae in the absence of the core centriole proteins. Our live imaging analysis showed that the MKS-5::mMaple3 fluorescence was enriched as puncta in the dendrite 68 ± 23 min after the dendrite elongates in WT animals (Fig 5A; N = 54). By contrast, MKS-5::mMaple3 did not form any puncta in 29% of sas-5-sg conditional mutants after the time-lapse recording for 2–3 h (Mean ± S.D.; N = 49; the representative images and movie are shown in Fig 2G and I, and in Movie EV2). Although 24% of the sas-5-sg animals eventually formed the MKS-5 fluorescence puncta at the dendrite tip, the process was severely retarded, taking 125 ± 33 min (Mean ± SD; N = 49; the representative images and movies are shown in Fig 2H and I, and in Movie EV2). The delayed formation of MKS-5 puncta in 24% of sas-5-sg conditional mutants is likely because residual SAS-5 builds a deformed centriole that is not as good at templating the cilium. Conditional mutations of sas-5 may not completely remove SAS-5 as it is essential for cell division and other events before ciliogenesis, and the absence of GFP::SAS-5 fluorescence in sas-5-sg animals may not indicate the complete loss of SAS-5, making it difficult to examine the role of residual SAS-5 in partial ciliogenesis. We cannot exclude the possibility that cells may form cilia without centrioles but not very efficiently. Together, these data indicate that centriolar proteins are essential for ciliogenesis in C. elegans. Centriole translocation depends on the cytoplasmic dynein-1 We next investigated the mechanism underlying centriole translocation along the dendrite. To explore microtubule polarity in the developing dendrites of L1 larvae, we constructed the microtubule plus-end binding protein EBP-2::GFP knock-in strain. The fluorescence of EBP-2::GFP appeared as comet-like streaks in the C. elegans embryos and in adult somatic cells (Movie EV3). As shown in the kymograph in Fig 3A, 80% of the EBP-2::GFP comets moved away from the growth cone to the cell body at 0.22 ± 0.11 μm/s, whereas the other 20% of the EBP-2::GFP comets moved in the opposite direction with a speed of 0.22 ± 0.11 μm/s, indicating that dendritic microtubules mostly emanate from the tip of the dendrite (Mean ± SD; N = 223 tracks in 12 cells; the representative images are shown in Figs 3A and EV3A). Consistently, in sensory neurons of adult C. elegans, 94% of EBP-2::GFP moved from the cilium base toward the cell body (Hao et al, 2011). The orientation of microtubules and the unidirectional movement of centrioles along the dendrite suggest that a minus-end-directed microtubule motor protein might be involved. Importantly, by visualizing DHC-1 localization in a GFP::DHC-1 animal, we showed that DHC-1 was restricted within the dendrite and did not enter cilia (Fig 3B). We postulate that centriole translocation may be driven by dynein-1 along the dendritic microtubules. Figure 3. Centriole translocation requires cytoplasmic dynein-1 A. EBP-2::GFP protein dynamics in PQR neuron during ciliogenesis in L1 larva (top). A kymograph (left bottom) with corresponding cartoons (right bottom) showing 80% of EBP-2::GFP comets move from cilium to soma at the speed of 0.22 ± 0.11 μm/s (N = 223) (Movie EV3). B. Dynein motility in dendrites of ciliated neurons. A kymograph (right top) with corresponding cartoons (right bottom) showing the soma-to-cilium directed movement of dynein; scale bars, 2 μm, 10 s. C. Centriole movement defects in conditional mutants visualized by SAS-6::GFP; 21 ± 11% for dhc-1-sg (N = 152, P < 0.01), 11 ± 5% for dlc-1-sg (N = 92, P < 0.01), and 10 ± 5% for lis-1-sg (N = 76, P < 0.05). D–F. (D, F) Fluorescence time-lapse images and quantification of centriole positioning during ciliogenesis in Dynein-1 mutants. Among these cells, 30% show two immobile centrioles remaining in the soma (left), 9% have one centriole in the dendrite without continuous movement toward the dendrite tip (middle), and 4% contain centriole that underwent backward migration to the cell body (right) (N = 46). Note that at 0 min dendrite already extended more than 1 μm (Movie EV4). (E) The centriole displacement tracks in Dynein-1 mutants compared with wild-type animals; the quantification is shown in (F). Data information: Scale bars, 5 μm except for Fig 3B. Download figure Download PowerPoint Click here to expand this figure. Figure EV3. Dynein-1 is involved in regulating centriole translocation EBP-2::GFP protein dynamics in ciliated neurons in adult worms. A kymograph (middle) with corresponding cartoons (bottom) showing 100% of EBP-2::GFP comets moving from the cilium to the soma. Fluorescence time-lapse images and quantification of centriole positioning during ciliogenesis in dynein light-chain 8 mutants. Fluorescence time-lapse images and quantification of centriole positioning during ciliogenesis in lis-1-sg mutants. A gel showing the T7EI assay results for dhc-1-sg. Ciliary morphology (amphid, phasmid, and PQR neuron) in Dynein subunits conditional mutants visualized by OSM-6::GFP. The cilium length was reduced from 8.3 ± 0.2 μm in WT animals to 3.7 ± 0.4 μm in dhc-1-sg, 3.5 ± 0.4 μm in dlc-1-sg, or 0.6 ± 0.4 μm in lis-1-sg conditional mutants (N = 25–61, Mean ± SEM). Yellow asterisks indicate the location of ciliary base. Data information: Scale bars, 5 μm. Download figure Download PowerPoint To study the function of dynein-1 in centriole translocation, we examined the position of centrioles of PQR neuron in the dhc-1-sg conditional mutant strain. At the late L1 larval stage, centriole translocation was completed in the dendrite of WT PQR neurons. However, in 21 ± 11% of dhc-1-sg mutant animals (N = 152) that properly elongated their dendrites, no centrioles could be detected in the dendrite; instead, two centrioles were retained in the cell body. Conditional mutations of the dynein-1 light-chain LC8 or the associated subunit Lissencephaly protein LIS-1 caused the same defects of centriole translocation, with penetrance of 11 ± 5% for dlc-1-sg (N = 92) and 10 ± 5% for lis-1-sg (N = 76; Fig 3C). To address which aspects of centriole movement require dynein-1, we followed centriole behavior in dynein-1 conditional mutant animals. For these live cell imaging analyses, our time-lapse recording started when the dendrite was over 1.5 μm in length. Three patterns of centriole behavior indicated the effects of dynein-1 inhibition. In one pattern, 30% of cells retained two immobile centrioles in the soma, while the dendrite extended; in the second pattern, 9% of cells had one centriole in the elongating dendrite but this centriole did not show any contin
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