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Comprehensive Multiphase (CMP) NMR Monitoring of the Structural Changes and Molecular Flux Within a Growing Seed

2017; American Chemical Society; Volume: 65; Issue: 32 Linguagem: Inglês

10.1021/acs.jafc.7b02421

1520-5118

Blythe Fortier‐McGill, Rudraksha Dutta Majumdar, Leayen Lam, Ronald Soong, Yalda Liaghati-Mobarhan, Andre Sutrisno, Ries de Visser, Myrna J. Simpson, Heather L. Wheeler, Malcolm M. Campbell, A. Gorissen, André J. Simpson,

Food composition and properties

A relatively recent technique termed comprehensive multiphase (CMP) NMR spectroscopy was used to investigate the growth and associated metabolomic changes of 13C-labeled wheat seeds and germinated seedlings. CMP-NMR enables the study of all phases in intact samples (i.e., liquid, gel-like, semisolid, and solid), by combining all required electronics into a single NMR probe, and can be used for investigating biological processes such as seed germination. All components, from the most liquid-like (i.e., dissolved metabolites) to the most rigid or solid-like (seed coat) were monitored in situ over 4 days. A wide range of metabolites were identified, and after 96 h of germination, the number of metabolites in the mobile phase more than doubled in comparison to 0 h (dry seed). This work represents the first application of CMP-NMR to follow biological processes in plants.