Produção Nacional
Revisado por pares
Photodynamic therapy with high-power LED mediated by erythrosine eliminates Enterococcus faecalis in planktonic forms
2017; Elsevier BV; Volume: 19; Linguagem: Inglês
10.1016/j.pdpdt.2017.07.007
ISSN1873-1597
AutoresAlberto Sabin Moura Borba, Sângela Maria da Silva Pereira, Mellyna Cavalcante Mendes Borba, Marco Aurélio Benini Paschoal, Rudys Rodolfo de Jesus Tavarez, Cláudia de Castro Rizzi, Meire Coelho Ferreira, Etevaldo Matos Maia Filho,
Tópico(s)Laser Applications in Dentistry and Medicine
ResumoThe failure of endodontic treatment is linked to the presence of microorganisms, particularly Enterococcus faecalis, in the root canals. This study evaluated the effectiveness of photodynamic therapy (PDT) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of E. faecalis. Bacterial suspensions of E. faecalis were adjusted and then mixed in a 1:1 proportion, in triplicate, in treatment groups by varying the length of irradiation time (120 and 240 s) and the molarity of the erythrosine (5 and 10 μM). In order to verify the post-treatment bactericidal effect, a count of the viable bacteria was performed (CFU mL−1) and transformed into Log10 CFU. The one-way ANOVA with Tukey post-hoc test was applied to check for differences between the groups. The bacteria were completely eradicated in the groups that used PDT with 5 μM 240 s, 10 μM 120 s and 10 μM 240 s (p ≪ 0.001). The effect of the PDT 5 μM 120 s group was significant (p ≪ 0.05) in comparison with the groups using only light or only erythrosine. Positive control (exposure to 2.5% NaClO for 120 and 240 s) completely eradicated E. faecalis. The negative control (PBS) did not alter the quantities of E. faecalis CFU with 9.605 Log10 CFU at 120 s and 9.621 Log10 CFU at 240 s. PDT with erythrosine in a concentration of 10 μM and high-power LED is capable of totally eliminating E. faecalis in planktonic suspension.
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