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Effect of feather meal as proteic feeder on combi-CLEAs preparation for grape juice clarification

2017; Elsevier BV; Volume: 62; Linguagem: Inglês

10.1016/j.procbio.2017.07.015

1873-3298

Daniela Goetze, Eliara F. Foletto, Halisson B. da Silva, Vitória C.C. Silveira, Lucas Dal Magro, Rafael C. Rodrigues,

Biofuel production and bioconversion

This work describes the preparation of combi-CLEAs of pectinases using feather meal as novel proteic feeder. Initially, four solvents were tested as precipitant agents, and ethanol was chosen as the best solvent. Subsequently, a 23 factorial design was carried out to define the optimal conditions for combi-CLEAs preparation, which were set as 110 mM of glutaraldehyde, 0.66 mg protein mL−1 and 5 h of reaction time. Then, the effect of BSA or feather meal (FM) as proteic feeders was evaluated. Combi-CLEAs, combi-CLEAs-BSA and combi-CLEAs-FM were characterized and evaluated by their activity recovery, optimal pH and temperature, operational and thermal stabilities and e effectiveness on juice clarification. The addition of feather meal increased two-fold the activity recovery compared to BSA. The thermal stability was similar for all combi-CLEAs, providing stabilization factors of 1.12, 1.48 and 1.53, respectively, for the combi-CLEA, combi-CLEA-FM and combi-CLEA-BSA, compared to free enzyme. CLEAs presented turbidity reduction near to 50%, 2.5-fold than soluble enzyme. Feather meal was used for the first time as proteic feeder for CLEA preparations and appears to be a good, and low price, alternative.