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Nanofat-derived stem cells with platelet-rich fibrin improve facial contour remodeling and skin rejuvenation after autologous structural fat transplantation

2017; Impact Journals LLC; Volume: 8; Issue: 40 Linguagem: Inglês

10.18632/oncotarget.19721

1949-2553

Hua Wei, Shixing Gu, Yi-Dan Liang, Zhi-Jie Liang, Hai Chen, Mao-Guang Zhu, Fang-Tian Xu, Ning He, Xiao-juan Wei, Hongmian Li,

Reconstructive Surgery and Microvascular Techniques

// Hua Wei 1, * , Shi-Xing Gu 2, * , Yi-Dan Liang 3, * , Zhi-Jie Liang 4 , Hai Chen 4 , Mao-Guang Zhu 4 , Fang-Tian Xu 5 , Ning He 6 , Xiao-Juan Wei 7 and Hong-Mian Li 6 1 Department of Endocrinology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China 2 Department of Burns & Plastic Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China 3 Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 4 Department of Gland Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 5 Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China 6 Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 7 Department of Urinary Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China * These authors have contributed equally to this work Correspondence to: Hong-Mian Li, email: 18022081568@163.com Keywords: nanofat, nanofat-derived stem cells, stromal vascular fraction, platelet-rich fibrin, structural fat transplantation Received: February 10, 2017 Accepted: June 19, 2017 Published: July 31, 2017 ABSTRACT Traditional autologous fat transplantation is a common surgical procedure for treating facial soft tissue depression and skin aging. However, the transplanted fat is easily absorbed, reducing the long-term efficacy of the procedure. Here, we examined the efficacy of nanofat-assisted autologous fat structural transplantation. Nanofat-derived stem cells (NFSCs) were isolated, mechanically emulsified, cultured, and characterized. Platelet-rich fibrin (PRF) enhanced proliferation and adipogenic differentiation of NFSCs in vitro . We then compared 62 test group patients with soft tissue depression or signs of aging who underwent combined nanofat, PRF, and autologous fat structural transplantation to control patients (77 cases) who underwent traditional autologous fat transplantation. Facial soft tissue depression symptoms and skin texture were improved to a greater extent after nanofat transplants than after traditional transplants, and the nanofat group had an overall satisfaction rate above 90%. These data suggest that NFSCs function similarly to mesenchymal stem cells and share many of the biological characteristics of traditional fat stem cell cultures. Transplants that combine newly-isolated nanofat, which has a rich stromal vascular fraction (SVF), with PRF and autologous structural fat granules may therefore be a safe, highly-effective, and long-lasting method for remodeling facial contours and rejuvenating the skin.