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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Differing energy densities with laser 670 nm InGaP controls inflammation and collagen reorganization in burns

2017; Elsevier BV; Volume: 43; Issue: 7 Linguagem: Inglês

10.1016/j.burns.2017.04.008

ISSN

1879-1409

Autores

Luís Angelo Ozan Maligieri, Lia Mara Grosso Neves, Driele Talita de Morais, Rayane Ferreira Domingues, Andréa Aparecida de Aro, Edson Rosa Pimentel, Maria Esméria Corezola do Amaral, Marcelo Augusto Marretto Esquisatto, Gláucia Maria Tech dos Santos, Fernanda Aparecida Sampaio Mendonça,

Tópico(s)

Wound Healing and Treatments

Resumo

This study compared different energy densities of laser on second degrees burns in rats aiming to determine the most effective dosimetry in stimulation of the healing process.Burns were induced in the dorsal skin of 54 animals divided into three groups (n: 18): 1-without treatment; 2-irradiated lesions by the Indium Gallium Phosphide (InGaP) 670nm (4.93J/cm2) laser; 3-irradiated lesions by the InGaP-670nm (9.86J/cm2) laser. Samples were collected on the 2, 10 and 18 days after injury for structural, morphometry, biochemical analysis and Western blotting.The energy densities examined were effective in significantly increasing the total number of fibroblasts and blood vessels and reduce the number of inflammatory cells particularly in irradiated lesions with 9.86J/cm2. This same energy density significantly increased the amount of GAGs (Glycosaminoglycans), decreased the TGF-β1 (Transforming Growth Factor β1) and increased the VEGF (Vascular and Endothelial Growth Factor) during the experimental period. This energy density also significantly increased the Collagen type I and decreased Collagen type III and the active isoform of metalloproteinase 9 (MMP-9).The energy density of 9.86J/cm2 was more effective in promoting cellular responses related to neoangiogenesis, decreasing inflammation and collagen fibers reorganization.

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