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Revisado por pares
First Report of Soft Rot in Kale Caused by Pectobacterium carotovorum subsp. brasiliensis in Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 12 Linguagem: Inglês
10.1094/pdis-02-17-0266-pdn
ISSN1943-7692
AutoresMárcia Ferreira Queiroz, Greecy Mirian Rodrigues Albuquerque, Marco Aurélio Siqueira da Gama, R. L. R. Mariano, Alessandra Jackeline Guedes de Moraes, Elineide Barbosa de Souza, Jarlyson Brunno Costa Souza, Cristiane Domingos da Paz, Ana Rosa Peixoto,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 101, No. 12First Report of Soft Rot in Kale Caused by Pectobacterium carotovorum subsp. brasiliensis in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Soft Rot in Kale Caused by Pectobacterium carotovorum subsp. brasiliensis in BrazilM. F. Queiroz, G. M. R. Albuquerque, M. A. S. Gama, R. L. R. Mariano, A. J. G. Moraes, E. B. Souza, J. B. Souza, C. D. Da Paz, and A. R. PeixotoM. F. Queiroz†Corresponding author: M. F. Queiroz; E-mail: E-mail Address: marcyabioagro@gmail.comhttp://orcid.org/0000-0002-8637-6863Search for more papers by this author, G. M. R. AlbuquerqueSearch for more papers by this author, M. A. S. GamaSearch for more papers by this author, R. L. R. MarianoSearch for more papers by this author, A. J. G. MoraesSearch for more papers by this author, E. B. SouzaSearch for more papers by this author, J. B. SouzaSearch for more papers by this author, C. D. Da PazSearch for more papers by this author, and A. R. PeixotoSearch for more papers by this authorAffiliationsAuthors and Affiliations M. F. Queiroz † , Universidade do Estado da Bahia, Juazeiro, 48905-680, Bahia, Brazil G. M. R. Albuquerque M. A. S. Gama R. L. R. Mariano A. J. G. Moraes E. B. Souza , Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil J. B. Souza C. D. Da Paz A. R. Peixoto , Universidade do Estado da Bahia, Juazeiro, 48905-680, Bahia, Brazil. Published Online:11 Oct 2017https://doi.org/10.1094/PDIS-02-17-0266-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Kale (Brassica oleracea var. acephala) is a widely grown leafy vegetable in Brazil. In November 2014 (rainfall 138.7 mm, average temperature 27.2°C, average relative humidity 56.5%), soft rot symptoms were detected on plants of kale cv. Manteiga in a 0.05 ha-field in Juazeiro County, State of Bahia, Brazil. Disease incidence was 40% and diseased plants presented leaf wilting and soft rot of stems with a foul smell. After insertion of a sterilized toothpick containing rotten kale stem tissue in symptomless pepper fruit and 36 h incubation in moist chamber, one strain (UNEB8) was obtained from symptomatic pepper fruits on peptone-casamino acid-glucose medium (CPG). In this medium, young colonies (24 h) of pectobacteria showed “broken glass” aspect when observed in stereoscope under oblique lighting (Kelman and Dickey 1995). Pathogenicity tests was twice conducted on seedlings (n = 5) and detached leaves (n = 5) of kale cv. Manteiga and Chinese cabbage cv. Michihilli (a very susceptible host), respectively, by injection of 10 µl of bacterial suspension (109 CFU/ml) of the isolate UNEB8. The seedlings were incubated at 30 ± 2°C and 62 ± 2% RH and the detached leaves at 28 ± 2°C and 65 ± 2% RH. Symptoms of soft rot were observed after 12 to 24 h of incubation and the same bacterium was reisolated from the inoculated tissues to complete Koch’s postulates. Negative control plants (n = 5) treated with sterile distilled water did not show symptoms. The strain UNEB8 was Gram-negative, has grown at 37°C in nutrient agar, was resistant to erythromycin, and macerated potato tubers. The Biolog Gen III System identified strain UNEB8 as a Pectobacterium sp. with a level of 69% of similarity. The molecular identification was performed from the sequencing of a 1,249-bp fragment of 16S rRNA gene (Darrasse et al. 1994). BLASTn analysis of 16S rDNA showed the sequence of strain UNEB8 (GenBank accession no. KX960101) shared 99.9% similarity to strain C18 (JF926718.1) of Pectobacterium carotovorum subsp. brasiliensis. Phylogenetic analysis was performed by the neighbor-joining method using the algorithm Kimura 2-parameters with 2,000 bootstraps. The UNEB8 strain was phylogenetically related with the strain C18 of P. carotovorum subsp. brasiliensis, which was obtained from Solanum tuberosum from Syria by Nabhan et al. (2012). Specific primers BR1f/L1r for identification of P. carotovorum subsp. brasiliensis (Duarte et al. 2004) were used and the strain UNEB8 produced a characteristic 322 bp fragment of this subspecies. The importance of this report relies on the register of an additional host for P. carotovorum subsp. brasiliensis, which until now was only reported in potato (Duarte et al. 2004) and zucchini (Moraes et al. 2017) in Brazil. To our knowledge, this is the first report of P. carotovorum subsp. brasiliensis as the causal agent of soft rot in kale in Brazil.References:Darrasse, A., et al. 1994. Appl. Environ. Microbiol. 60:1437. Crossref, ISI, Google ScholarDuarte, V., et al. 2004. J. Appl. Microbiol. 96:535. https://doi.org/10.1111/j.1365-2672.2004.02173.x Crossref, ISI, Google ScholarKelman, A., and Dickey, R. S. 1995. Page 76 in: Detection of Bacteria on Seed and Other Planting Material. APS Press, St. Paul, MN. Google ScholarMoraes, A. J. G., et al. 2017. Plant Dis. 101:379. https://doi.org/10.1094/PDIS-08-16-1168-PDN Link, ISI, Google ScholarNabhan, S., et al. 2012. Plant Pathol. 61:498. https://doi.org/10.1111/j.1365-3059.2011.02546.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 12 December 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 16 Nov 2017Published: 11 Oct 2017First Look: 1 Aug 2017Accepted: 27 Jul 2017 Pages: 2144-2144 Information© 2017 The American Phytopathological SocietyCited byChemical Composition, Antibacterial, Antioxidant and Enzyme Inhibitory Activities of the Essential Oil from Leaves of Psidium guajava L.12 April 2022 | Chemistry & Biodiversity, Vol. 19, No. 5Pectobacterium carotovorumCABI Compendium, Vol. CABI CompendiumCharacterization of Pectobacterium brasiliense strains from potato and vegetables in Israel30 August 2021 | Plant Pathology, Vol. 70, No. 9First Report of Pectobacterium brasiliense Causing Soft Rot on Brassica oleracea var. sabellica in Hawaii, United StatesGamze Boluk, Dario Arizala, Jordie Ocenar, Julie Mokwele, Joshua Silva, Shefali Dobhal, Jensen Uyeda, Anne M. Alvarez, and Mohammad Arif18 August 2020 | Plant Disease, Vol. 104, No. 10The Changing Face of Bacterial Soft-Rot DiseasesAnnual Review of Phytopathology, Vol. 56, No. 1
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