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P9 The effect of ethanolic extract of red betel vine leaves (Piper crocatum) to the livers and kidneys of DDY mice in acute and subchronic toxicity study

2017; Elsevier BV; Volume: 139; Linguagem: Inglês

10.1016/j.bcp.2017.06.010

1873-2968

Farida Juliantina Rachmawaty, Zaenuri Sabta Nugraha, Ika Fidianingsih, Mutia Amalina, Dita Widira,

Pharmacogenetics and Drug Metabolism

The ethanolic extract of red betel vine leaves (Piper crocatum) has been shown to exert antimycobacterial activities against Mycobacterium tuberculosis. As it takes a long time to treat tuberculosis, the safety of chronic administration of a drug requires evaluation. This study aims to determine the effects of red betel vine leaves ethanolic extract on the liver and kidneys of DDY mice in both acute and sub-chronic toxicity studies. Sixty mice were used throughout this study. In the acute toxicity study, mice were administrated orally with dosages of 1000, 500, 250, 125 mg/mL. The physical condition and behaviour of the mice, and incidence of death within the groups were recorded for the first 24 h. These observations were then continued for two weeks. In the subchronic toxicity study (90 days), mice were administrated orally with dosages of 50, 100, 200, 400 mg/kg b.w. The observations carried out in this study were similar to those recorded in the acute toxicity study. At the end of the study, mice were terminated by using cervical dislocation. The liver and kidneys were harvested for histopathological assessment. LD50 was obtained at 18 g/kg b.w in the acute toxicity study. The histopathology assessment results of mice given a therapeutic dose were normal. In the chronic toxicity study, the histopathology of kidneys was not significantly different from those of controls, however there was reversible hydropic degeneration of the liver in these animals. The effects of therapeutic doses of red betel vine leaves ethanolic extract on the liver and kidneys of DDY mice appear tolerable in both the acute and subchronic toxicity studies.