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First Report of Maize yellow mosaic virus Infecting Maize in Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 12 Linguagem: Inglês
10.1094/pdis-04-17-0569-pdn
ISSN1943-7692
AutoresMarcos César Gonçalves, Márcio Tadeu Godinho, D. M. T. Alves-Freitas, Arvind Varsani, Simone G. Ribeiro,
Tópico(s)Plant Disease Management Techniques
ResumoHomePlant DiseaseVol. 101, No. 12First Report of Maize yellow mosaic virus Infecting Maize in Brazil Previous DISEASE NOTES OPENOpen Access licenseFirst Report of Maize yellow mosaic virus Infecting Maize in BrazilM. C. Gonçalves, M. Godinho, D. M. T. Alves-Freitas, A. Varsani, and S. G. RibeiroM. C. Gonçalves, M. Godinho, D. M. T. Alves-Freitas, A. Varsani, and S. G. Ribeiro†Corresponding author: S. G. Ribeiro; E-mail: E-mail Address: simone.ribeiro@embrapa.brAffiliationsAuthors and Affiliations M. C. Gonçalves , Instituto Biológico, Crop Protection Research Centre, São Paulo, SP, Brazil M. Godinho D. M. T. Alves-Freitas , Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil A. Varsani , The Biodesign Center for Fundamental and Applied Microbiomics, Center of Evolution and Medicine, School of Life sciences, Arizona State University, Tempe, AZ S. G. Ribeiro † , Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil. Published Online:13 Oct 2017https://doi.org/10.1094/PDIS-04-17-0569-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Maize (Zea mays) is a staple crop for human consumption and widely used as a livestock feed worldwide. Therefore, potential new diseases affecting this crop are of major concern. In May 2013, yellow mosaic and dwarfing symptoms were observed in several maize plants in a field located in the rural area of Casa Branca (21°46′26″ S, 47°5′9″ W), São Paulo state (southeastern Brazil). Symptomatic leaves were sampled, and total RNA was isolated using Trizol reagent and further purified with PureLink RNA Mini Kit spin columns (Invitrogen, U.S.A.). A library was prepared with an Illumina TruSeq Stranded Total RNA sample preparation kit with Ribo-Zero Plant and sequenced on an Illumina Hiseq 2500 platform at Macrogen Inc, South Korea. De novo assembly using Abyss 1.9 with K-mer = 64 yielded a contig of 1,183 nt with high similarities (92 to 93%) to several isolates sequences of Maize yellow mosaic virus (MaYMV), a putative new member of the genus Polerovirus, family Luteoviridae, recently reported in China (Chen et al. 2016). A MaYMV sequence (GenBank KU179221) was used as a scaffold for posterior mapping of Illumina short sequencing reads using Bowtie 2 (Langmead and Salzberg 2012) and a final contig of 5,637 nt was generated and designated MaYMV-SP1. Based on the assembled contig, we designed three primer pairs to conduct RT-PCR, to amplify a fragment of 744 nt from ORF1 (P1F: 5′-CCTGCTTGTCCAACACAACG-3′ and P1R: 5′-TCCGTTTTGTTTTCGCGGAC-3′), a 1,942-nt section from ORF2 (P2F: 5′-AGCAAGCAGTGTTCTCCCTC-3′ and P2R: 5′-GCCCCGTCTACCTATTTCGG-3′), and a 1,459-nt fragment from ORF3 (P3F: 5′-GGCGGAATTCTCAAGGCCTA-3′ and P3R: 5′-CAGTCAGCGTTGGGGTTTTG-3′). RT-PCR using the total RNA isolated from the sequenced field-sample yielded three fragments of the expected sizes, which were cloned into pCR 2.1-TOPO vector (Thermo Fisher, U.S.A.) and Sanger sequenced. The sequences of the fragments from ORF1 and ORF2 were 100% identical to the NGS contig, and the sequence from the region of ORF3 shared a sequence identity of 98.8%. RT-PCR with primer pair P1F/P1R was used to amplify MaYMV from two additional samples, showing similar symptoms, also collected in Casa Branca district, in 2017. Sequencing of the amplicons confirmed the presence of MaYMV in plants from a distinct maize field. MaYMV-SP1 (KY940544) has a genomic organization typical of the poleroviruses with six open reading frames (ORFs) and three untranslated regions (UTRs). Using SDTv 1.2 program (Muhire et al. 2014), we found that MaYMV-SP1 shares ∼96% nucleotide pairwise identity, with MaYMV isolates (KU179221, KU248489–490, KU291099–108) and with Maize yellow dwarf virus-RMV2 (KT992824) all identified from China (Wang et al. 2016). Furthermore, MaYMV-SP1 from Brazil shares 75% pairwise identity with the polerovirus Maize yellow dwarf virus-RMV (KC921392) from the U.S.A. (Krueger et al. 2013). To our best knowledge, this is the first report of MaYMV, a tentative member of the genus Polerovirus infecting maize in Brazil. The economic importance of MaYMV infection in maize is currently not known. However, the widespread presence of putative aphid vectors, e.g., Rhopalosiphum maidis, in corn fields in Brazil, implies that MaYMV might represent a potential threat to maize production. Studies are in progress to better understand the incidence, distribution, and possible interactions of MaYMV with other known maize-infecting viruses in Brazil.References:Chen, S., et al. 2016. Viruses 8:120. https://doi.org/10.3390/v8050120 Crossref, ISI, Google ScholarKrueger, E. N., et al. 2013. Front. Microbiol. 4:1. https://doi.org/10.3389/fmicb.2013.00205 Crossref, ISI, Google ScholarLangmead, B., and Salzberg, S. 2012. Nat. Methods 9:357. https://doi.org/10.1038/nmeth.1923 Crossref, ISI, Google ScholarMuhire, B. M., et al. 2014. PLoS One 9:e108277. https://doi.org/10.1371/journal.pone.0108277 Crossref, ISI, Google ScholarWang, F., et al. 2016. Plant Dis. 100:1508. https://doi.org/10.1094/PDIS-11-15-1259-PDN Link, ISI, Google ScholarThis work was supported by grants from Embrapa and CNPq. M. Godinho and D. M. T. Alves-Freitas are recipients of postdoctoral fellowships from CNPq.DetailsFiguresLiterature CitedRelated Vol. 101, No. 12 December 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 16 Nov 2017Published: 13 Oct 2017First Look: 1 Aug 2017Accepted: 29 Jul 2017 Page: 2156 Information© 2017 The American Phytopathological SocietyCited byFirst Report of Maize Yellow Mosaic Virus Causing Maize Reddening in Henan, ChinaY. J. Shi, X. Y. Han, Q. L. Li, X. M. Liu, L. L. Yang, H. Wang, X. L. Jiang, H. L. Li, X. Yang, and Y. Shi30 October 2022 | Plant Disease, Vol. 106, No. 12Development of an RT-LAMP assay for the detection of maize yellow mosaic virus in maizeJournal of Virological Methods, Vol. 300Maize yellow mosaic virusCABI Compendium, Vol. CABI CompendiumMaize Yellow Mosaic Virus Interacts with Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus in Mixed Infections, But Does Not Cause Maize Lethal NecrosisLucy R. 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