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First Report of Banana bunchy top virus in Heliconia spp. on Hawaii

2017; American Phytopathological Society; Volume: 101; Issue: 12 Linguagem: Inglês

10.1094/pdis-02-17-0205-pdn

1943-7692

Islam Hamim, J. C. Green, Wayne B. Borth, Michael J. Melzer, Yanan Wang, John Hu,

Plant and Fungal Interactions Research

HomePlant DiseaseVol. 101, No. 12First Report of Banana bunchy top virus in Heliconia spp. on Hawaii PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Banana bunchy top virus in Heliconia spp. on HawaiiI. Hamim, J. C. Green, W. B. Borth, M. J. Melzer, Y. N. Wang, and J. S. HuI. Hamim†Corresponding author: I. Hamim; E-mail: E-mail Address: hamim@hawaii.edu, J. C. Green, W. B. Borth, M. J. Melzer, Y. N. Wang, and J. S. HuAffiliationsAuthors and Affiliations I. Hamim † J. C. Green W. B. Borth M. J. Melzer Y. N. Wang J. S. Hu , Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822. Published Online:11 Oct 2017https://doi.org/10.1094/PDIS-02-17-0205-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Banana bunchy top virus (BBTV) infects Musa spp. and causes banana bunchy top disease (BBTD), one of the most significant constraints to banana (Musa spp.) production worldwide (Xie and Hu 1995). The virus is transmitted by banana aphids (Pentalonia spp.), which can also feed on heliconia (Heliconia spp.) (Watanabe et al. 2013). Heliconias, native to the tropical Americas, are common landscape plants in Hawaii and have naturalized in some disturbed areas and forests. If heliconias are hosts of BBTV, they may potentially pose a significant threat to banana production in Hawaii. In July 2016, heliconia (Heliconia aurantiaca) plants with chlorotic leaves and green flecking of the veins were observed in a community garden in Honolulu, HI (21.2851°N, 157.8280°W). Fifteen symptomatic samples were tested for BBTV by PCR assays using virus-specific primers BBTV-RT1F (5′-ACCAGCCGACTACATGTCTG-3′) and BBTV-RT2R (5′-TCCTCAACAC GGTTGTCTTC-3′). The expected 155-bp fragment of the partial capsid protein (CP) gene was amplified from eight of the 15 samples. These samples also tested positive in triple-antibody sandwich (TAS)-ELISA for BBTV (Agdia, Elkhart, IN), confirming the presence of the virus. Thirty-two additional heliconia samples with similar symptoms were collected from the same location and 22 tested positive in seven PCR tests using two different sets of BBTV-specific primer pairs: Rep-gene-specific primers BBTV-RepF1 (5′-CCATCAACAATCCCACAACA-3′) and BBTV-RepR1 (5′-ACAGTATGACCGCGCGCTTCTT-3′); and CP-specific primers BBTV-HAF1 (5′-TCCGAAGAATCCATCAAGA-3′) and BBTV-HAR1 (5′-ACACGGTTGTCTTCCTCAAA-3′). The positive PCR and ELISA results from symptomatic heliconia samples were weaker than those produced from BBTV-infected banana in both assays. To further verify the results, the 208-bp amplicons of the Rep sequence and the 401-bp amplicons of the CP sequence were cloned into pGEM-T-Easy (Promega, Madison, WI) and sequenced. The 208-bp sequence (accession no. KY322773) and the 401-bp sequence (KY322774) shared 98 to 100% and 98 to 99% nucleotide sequence identities, respectively, to the Hawaiian and other Pacific isolates of BBTV. In addition, 14 heliconia samples (H. aurantiaca) with similar BBTV-like symptoms were collected from another location in Honolulu (21.2711°N, 157.8215°W) and assayed for BBTV by PCR and ELISA. Four of the 14 samples tested positive for BBTV by ELISA and PCR sequencing, with intensities similar to those noted above. To our knowledge, this is the first report of BBTV infecting Heliconia sp. in Hawaii. Further study is needed to determine whether banana aphids can transmit BBTV from infected heliconia to healthy banana plants.References:Watanabe, S., et al. 2013. Viruses 5:758. https://doi.org/10.3390/v5020758 Crossref, ISI, Google ScholarXie, W. S., and Hu, J. S. 1995. Phytopathology 85:339. https://doi.org/10.1094/Phyto-85-339 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 12 December 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 16 Nov 2017Published: 11 Oct 2017First Look: 9 Aug 2017Accepted: 4 Aug 2017 Page: 2153 Information© 2017 The American Phytopathological SocietyCited byPreserving plant samples from remote locations for detection of RNA and DNA viruses25 August 2022 | Frontiers in Microbiology, Vol. 13Musa Germplasm A and B Genomic Composition Differentially Affects Their Susceptibility to Banana Bunchy Top Virus and Its Aphid Vector, Pentalonia nigronervosa29 April 2022 | Plants, Vol. 11, No. 9Recent distribution and diversity analysis on banana bunchy top virus of banana and alternative host in Indonesia25 June 2021 | Tropical Plant Pathology, Vol. 46, No. 5Heliconia spp.6 June 2020