Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer
2017; Lippincott Williams & Wilkins; Volume: 199; Issue: 1 Linguagem: Inglês
10.1016/j.juro.2017.08.001
ISSN1527-3792
AutoresEva Shrestha, James R. White, Shu-Han Yu, İbrahim Kulaç, Onur Ertunç, Angelo M. De Marzo, Srinivasan Yegnasubramanian, Leslie A. Mangold, Alan W. Partin, Karen S. Sfanos,
Tópico(s)Urinary Bladder and Prostate Research
ResumoNo AccessJournal of UrologyAdult Urology1 Jan 2018Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer Eva Shrestha, James R. White, Shu-Han Yu, Ibrahim Kulac, Onur Ertunc, Angelo M. De Marzo, Srinivasan Yegnasubramanian, Leslie A. Mangold, Alan W. Partin, and Karen S. Sfanos Eva ShresthaEva Shrestha Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , James R. WhiteJames R. White Resphera Biosciences, Baltimore, Maryland Financial and/or other relationship with Resphera Biosciences. More articles by this author , Shu-Han YuShu-Han Yu Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Ibrahim KulacIbrahim Kulac Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Onur ErtuncOnur Ertunc Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Angelo M. De MarzoAngelo M. De Marzo Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Srinivasan YegnasubramanianSrinivasan Yegnasubramanian Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Leslie A. MangoldLeslie A. Mangold Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , Alan W. PartinAlan W. Partin Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author , and Karen S. SfanosKaren S. Sfanos Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.08.001AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Materials and Methods: Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Results: Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. Conclusions: To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer. References 1 : The microbiome of the urinary tract—a role beyond infection. Nat Rev Urol2015; 12: 81. Google Scholar 2 : The female urinary microbiome: a comparison of women with and without urgency urinary incontinence. MBio2014; 5: e01283. Google Scholar 3 : Urine is not sterile: use of enhanced urine culture techniques to detect resident bacterial flora in the adult female bladder. J Clin Microbiol2014; 52: 871. Google Scholar 4 : The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults. Front Cell Infect Microbiol2013; 3: 41. Google Scholar 5 : Characteristic male urine microbiomes associate with asymptomatic sexually transmitted infection. PLoS One2010; 5: e14116. Google Scholar 6 : Bacterial communities of the coronal sulcus and distal urethra of adolescent males. PLoS One2012; 7: e36298. Google Scholar 7 : Urinary microbiome and cytokine levels in women with interstitial cystitis. Obstet Gynecol2017; 129: 500. Google Scholar 8 : Structure, function and diversity of the healthy human microbiome. Nature2012; 486: 207. Google Scholar 9 : Infections and inflammation in prostate cancer. Am J Clin Exp Urol2013; 1: 3. Google Scholar 10 : Inflammation in prostate carcinogenesis. Nat Rev Cancer2007; 7: 256. Google Scholar 11 : Prostate cancer and inflammation: the evidence. Histopathology2012; 60: 199. Google Scholar 12 Mani RS, Amin MA, Li X et al: Inflammation-induced oxidative stress mediates gene fusion formation in prostate cancer. Cell Rep 17: 2620. Google Scholar 13 : Prostatic inflammation enhances basal-to-luminal differentiation and accelerates initiation of prostate cancer with a basal cell origin. Proc Natl Acad Sci U S A2014; 111: E592. Google Scholar 14 : Intermediate cells in human prostate epithelium are enriched in proliferative inflammatory atrophy. Am J Pathol2003; 162: 1529. Google Scholar 15 : Sexually transmitted infections and prostatic inflammation/cell damage as measured by serum prostate specific antigen concentration. J Urol2006; 175: 1937. Link, Google Scholar 16 : A molecular analysis of prokaryotic and viral DNA sequences in prostate tissue from patients with prostate cancer indicates the presence of multiple and diverse microorganisms. Prostate2008; 68: 306. Google Scholar 17 : Propionibacterium acnes associated with inflammation in radical prostatectomy specimens: a possible link to cancer evolution?. J Urol2005; 173: 1969. Link, Google Scholar 18 : Multilocus sequence typing (MLST) analysis of Propionibacterium acnes isolates from radical prostatectomy specimens. Prostate2013; 73: 770. Google Scholar 19 : A mouse model of chronic prostatic inflammation using a human prostate cancer-derived isolate of Propionibacterium acnes. Prostate2013; 73: 1007. Google Scholar 20 : Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus (the Streptococcus milleri group): association with different body sites and clinical infections. J Clin Microbiol1992; 30: 243. Google Scholar 21 : Gram-positive uropathogens, polymicrobial urinary tract infection, and the emerging microbiota of the urinary tract. Microbiol Spectr2016; 410.1128/microbiolspec.UTI. Crossref, Google Scholar 22 : Actinobaculum schaalii: review of an emerging uropathogen. J Infect2012; 64: 260. Google Scholar 23 : Varibaculum cambriense infections in Hong Kong, China, 2006. Emerg Infect Dis2009; 15: 1137. Google Scholar 24 : Two cases of urinary tract infection caused by Propionimicrobium lymphophilum. J Clin Microbiol2015; 53: 3077. Google Scholar 25 : An evaluation of PCR primer sets used for detection of Propionibacterium acnes in prostate tissue samples. Prostate2008; 68: 1492. Google Scholar 26 : Inflammation and benign prostatic hyperplasia. Urol Clin North Am2008; 35: 109. Google Scholar 27 : Chronic inflammation in the pathogenesis of benign prostatic hyperplasia. Int J Androl2010; 33: 475. Google Scholar 28 : The role of chronic prostatic inflammation in the pathogenesis and progression of benign prostatic hyperplasia (BPH). BJU Int2013; 112: 432. Google Scholar © 2018 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byOresta B, Braga D, Lazzeri M, Frego N, Saita A, Faccani C, Fasulo V, Colombo P, Guazzoni G, Hurle R and Rescigno M (2020) The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease StageJournal of Urology, VOL. 205, NO. 1, (86-93), Online publication date: 1-Jan-2021.Assimos D (2019) Re: Antibiotic Use and Risk of Incident Kidney Stones in Female NursesJournal of Urology, VOL. 203, NO. 3, (459-459), Online publication date: 1-Mar-2020. Volume 199Issue 1January 2018Page: 161-171Supplementary Materials Advertisement Copyright & Permissions© 2018 by American Urological Association Education and Research, Inc.Keywordsurinary tractbacteriamicrobiotaprostatic neoplasmsinflammationMetricsAuthor Information Eva Shrestha Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author James R. White Resphera Biosciences, Baltimore, Maryland Financial and/or other relationship with Resphera Biosciences. More articles by this author Shu-Han Yu Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Ibrahim Kulac Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Onur Ertunc Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Angelo M. De Marzo Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Srinivasan Yegnasubramanian Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Leslie A. Mangold Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Alan W. Partin Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Karen S. Sfanos Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland More articles by this author Expand All Advertisement PDF downloadLoading ...
Referência(s)