Gamma Glutamyl Transferase
1978; Karger Publishers; Volume: 23; Issue: 6 Linguagem: Inglês
10.1159/000458605
ISSN2504-2564
AutoresEtienne Malvoisin, M. Mercier, Marcel Roberfroid,
Tópico(s)Genomics, phytochemicals, and oxidative stress
Resumoγ-Glutamyl transferase (γ-GT) is a key catalyst in the metabolism of glutathione. Its activity in the rat liver is usually very low but it increases significantly during the process of chemical hepatocarcinogenesis. A new radiochemical assay is reported which measures the amount of 3H-aniline liberated from γ-glutamyl-3H-anilide. This assay is highly specific and no biological substances interferes. By applying this new assay to the analysis of the subcellular distribution of the rat liver γ-GT, it is shown that it distributes between two pools, one which corresponds to a membrane-bound enzyme, the other to a soluble enzyme. The subcellular distribution of γ-GT is discussed by comparison with the patterns of distribution of classical marker enzymes.
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