Produção Nacional
Revisado por pares
Syzygium cumini leaf extract inhibits LDL oxidation, but does not protect the liproprotein from glycation
2017; Elsevier BV; Volume: 210; Linguagem: Inglês
10.1016/j.jep.2017.08.033
ISSN1872-7573
AutoresMatheus Mülling dos Santos, Alessandro de Souza Prestes, Gabriel Teixeira de Macedo, Assis Ecker, Rômulo Pillon Barcelos, Aline Augusti Boligon, Diego de Souza, Andreza Fabro de, João Batista Teixeira da Rocha, Nilda Vargas Barbosa,
Tópico(s)Phytochemicals and Antioxidant Activities
ResumoSyzygium cumini (L.) Skeels is a plant widely used in folk medicine to treat diabetes mellitus (DM). The tea from its leaves is frequently used by diabetics for lowering hyperglycemia. There is a close relationship between DM and atherosclerosis, a chronic immuno-inflammatory disease, were the early stages encompass oxidative and glycative modifications in the structure of low density lipoprotein (LDL).To investigate the potential protective effects of aqueous-leaf extract from Syzygium cumini (S.cExt) against CuSO4-induced oxidation and methylglyoxal (MG)-induced glycation of human LDL in vitro.LDL oxidative changes were evaluated by measuring conjugated dienes (CD) formation, thiobarbituric acid reactive substances (TBARS) levels, quenching of tryptophan (Trp) fluorescence and structural modifications in LDL particle. In LDL glycated by MG (glyLDL), we determined the levels of fluorescent advanced glycation end products (AGEs) and mobility by agarose gel electrophoresis.S.cExt blocked oxidative events induced by CuSO4 in human LDL, plasma and serum. Fourier transform infrared spectroscopy (FT-IR) revealed that specific regions of apoB100 were oxidized by CuSO4 in human LDL and that S.cExt reduced these oxidations. Unlike, the increased AGEs levels and eletrophoretic mobility observed in LDL MG-glycated were not modified by S.cExt.The findings herein indicate that S.cExt could be tested in atherogenesis models as potential protective agent against LDL oxidation.
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