Vinculin forms a directionally asymmetric catch bond with F-actin
2017; American Association for the Advancement of Science; Volume: 357; Issue: 6352 Linguagem: Inglês
10.1126/science.aan2556
ISSN1095-9203
AutoresDerek L. Huang, Nicolas A. Bax, Craig Buckley, William I. Weis, Alexander R. Dunn,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoMaking the right catch Tension reveals cryptic vinculin-binding sites on α-catenin and talin at cadherin-based cell-cell and integrin-based cell-matrix adhesions, respectively. The enrichment of vinculin at cellular adhesions is thus an indicator of load-induced reinforcement of the cytoskeletal linkage. Huang et al. used a single-molecule optical trap assay to measure the binding lifetimes of vinculin to single actin filaments under load. The vinculin-F-actin interaction formed a directional catch bond—one that is very weak at low force but that greatly increases in lifetime with increasing force. This explains vinculin's role as a reinforcing linker at both cell-cell and cell-matrix adhesions. Science , this issue p. 703
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