Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus
2017; Public Library of Science; Volume: 12; Issue: 8 Linguagem: Inglês
10.1371/journal.pone.0183824
ISSN1932-6203
AutoresЕ.К. Петрова, Evgeniya Pechnikova, Maryia Mishyna, Tatiana I. Manukhova, Olga S. Sokolova, Nikolai A. Nikitin, J.G. Atabekov, О. В. Карпова,
Tópico(s)Animal Virus Infections Studies
ResumoPlant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.
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