Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care Testing
2017; American Chemical Society; Volume: 2; Issue: 9 Linguagem: Inglês
10.1021/acssensors.7b00495
ISSN2379-3694
AutoresXiang Ren, Junrong Yan, Dan Wu, Qin Wei, Yakun Wan,
Tópico(s)Advanced Biosensing Techniques and Applications
ResumoAlzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD.
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