Voltar
Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Efficient differentiation of Corynebacterium striatum , Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers

2017; Elsevier BV; Volume: 142; Linguagem: Inglês

10.1016/j.mimet.2017.09.002

ISSN

1872-8359

Autores

Carolina S. Santos, Juliana Nunes Ramos, Verônica Viana Vieira, Carina S. Pinheiro, Roberto Meyer, Neuza Maria Alcântara‐Neves, Rommel Thiago Jucá Ramos, Artur Silva, Raphael Hirata, Liza Felicori, Carlos Ruiz de Alegría Puig, Jesús Navas, Vasco Azevedo, Ana Luíza Mattos‐Guaraldi, Luis G. C. Pacheco,

Tópico(s)

Infective Endocarditis Diagnosis and Management

Resumo

A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

Referência(s)