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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column

2017; Elsevier BV; Volume: 1518; Linguagem: Inglês

10.1016/j.chroma.2017.08.071

ISSN

1873-3778

Autores

Meire Ribeiro da Silva, Ole Kristian Brandtzaeg, Tore Vehus, Fernando Mauro Lanças, Steven Ray Wilson, Elsa Lundanes,

Tópico(s)

Mass Spectrometry Techniques and Applications

Resumo

An open tubular (OT) sample preparation/separation platform was developed. A multi-channel polymer layer open tubular (mPLOT) solid phase extraction (SPE) column was prepared by wall-coating the 126 channels (8μm inner diameter (ID) each) of a crystal fiber capillary with an organic polymer, namely poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB). The mPLOT SPE was coupled on-line with a 10μm×2m poly(styrene-co-divinylbenzene) (PS-DVB) OT liquid chromatography column with nanospray mass spectrometry (OTLC-MS). Compared to using monolithic/particle-packed SPEs, mPLOT-SPE-OTLC allowed both fast loading and sufficient refocusing on the OT analytical column of small model compounds (sulfonamides≈300Da). Using automatic filtration/filter back-flushing (AFFL) plumbing, the mPLOT SPE column gave a constant and low back-pressure ≈35bar at 0.5μL/min. Surprisingly large sample volumes (10μL) were possible to be injected using a 12cm mPLOT.

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