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Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

2017; Nature Portfolio; Volume: 7; Issue: 1 Linguagem: Inglês

10.1038/s41598-017-11043-0

2045-2322

Ana Carolina Urbaczek, Paulo Augusto Gomes Carneiro Leão, Fayene Zeferino Ribeiro de Souza, Ana Afonso, Juliana Vieira Alberice, Luciana Teresa Dias Cappelini, Iracilda Zeppone Carlos, Emanuel Carrilho,

Microfluidic and Capillary Electrophoresis Applications

Abstract This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays ( i.e ., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.