First Report of Tomato chlorosis virus Infecting Tomato in Nigeria
2017; American Phytopathological Society; Volume: 102; Issue: 1 Linguagem: Inglês
10.1094/pdis-07-17-1099-pdn
ISSN1943-7692
AutoresIbrahim Umar Mohammed, A. M. Yakub, Ibrahim Yusuf, Aisha Muhammad, Jesús Navas‐Castillo, Elvira Fiallo‐Olivé,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 102, No. 1First Report of Tomato chlorosis virus Infecting Tomato in Nigeria PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato chlorosis virus Infecting Tomato in NigeriaI. U. Mohammed, A. M. Yakub, I. Yusuf, A. Muhammad, J. Navas-Castillo, and E. Fiallo-OlivéI. U. MohammedSearch for more papers by this author, A. M. YakubSearch for more papers by this author, I. YusufSearch for more papers by this author, A. MuhammadSearch for more papers by this author, J. Navas-CastilloSearch for more papers by this author, and E. Fiallo-Olivé†Corresponding author: E. Fiallo-Olivé; E-mail: E-mail Address: efiallo@eelm.csic.esSearch for more papers by this authorAffiliationsAuthors and Affiliations I. U. Mohammed A. M. Yakub I. Yusuf A. Muhammad , Kebbi State University of Science and Technology, Aliero (KSUSTA), Kebbi State, Nigeria J. Navas-Castillo E. Fiallo-Olivé † , Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Universidad de Málaga – Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain. Published Online:6 Nov 2017https://doi.org/10.1094/PDIS-07-17-1099-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In January and February 2017, symptoms of yellowing and leaf curling were observed in open-field Roma-type tomato (Solanum lycopersicum) plants in seven states (Kaduna, Kano, Katsina, Kebby, Jigawa, Sokoto, and Zamfara) of northern Nigeria. Almost 100% of the plants in the surveyed fields showed symptoms and were also heavily infested with the whitefly (Hemiptera: Aleyrodidae) Bemisia tabaci. Symptoms resembled those caused by Tomato chlorosis virus (ToCV), an emergent whitefly-transmitted virus found in many tropical, subtropical, and temperate countries worldwide (Navas-Castillo et al. 2011). Leaf samples were collected from 21 symptomatic plants, each from a different local government area, three per state (Giwa, Sabon Gari, and Zaria, KD; Bunkure, Garun Mallam, and Kura, KN; Funtua, Danja, and Bakori, KT; Birnin Kebbi, Jega, and Zuru, KE; Auyo, Kafin Hausa, and Hadejia, JI; Tambuwal, Shagari, and Wamako, SO; and Bakura, Talata Mafara, and Gusau, ZA). Total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, U.S.A.) from all samples and a healthy tomato leaf (cultivar Moneymaker) that was used as a negative control. All samples were analyzed by dot-blot hybridization using a digoxigenin-labeled RNA probe to the coat protein (CP) gene of ToCV (Fortes et al. 2012). Positive signal was obtained for all samples except for the negative control. Reverse-transcription PCR reactions were performed using specific primers for the detection of ToCV, MA396(+) (5′-TGGTCGAACAGTTTGAGAGC-3′) and MA397(–) (5′-TGAACTCGAATTGGGACAGA-3′), specific to the RNA-dependent RNA polymerase (RdRp) gene (ToCV RNA 1), and MA380(+) (5′-GTGAGACCCCGATGACAGAT-3′) and MA381(–) (5′-TACAGTTCCTTGCCCTCGTT-3′), specific to the CP gene (ToCV RNA 2), using avian myeloblastosis virus reverse transcription (Promega, Madison, WI, U.S.A.) and BIOTAQ (Bioline, London, U.K.). DNA fragments of the expected sizes (763 and 436 nt for RdRp and CP genes, respectively) were obtained for all symptomatic samples, thus supporting the presence of ToCV. The amplified products from two samples of the most distant states (Birnin Kebbi, KE and Hadejia, JI) were cloned in pGEM-T Easy vector (Promega) and sequenced (Macrogen, Seoul, South Korea). The two sequenced fragments of the RdRp gene (GenBank accession nos. MF459657 and MF459658) showed a nucleotide identity of 99.3% between them and the highest identity (99.2 to 99.4%) with ToCV isolates from Brazil (JQ952600) and Greece (EU284745) deposited in GenBank. The two sequenced fragments of the CP gene (MF459659 and MF459660) showed an identity of 100% between them and the highest identity (100%) with several ToCV isolates from China (KP217196), South Korea (KP114537), Greece (HG380090), the United States (HQ879842), and Tunisia (KC156614). To our knowledge, this is the first report of the presence of tomato chlorosis virus in Nigeria. In continental Africa, ToCV has been previously found only in northern Africa (Morocco, Tunisia, and Sudan) and South Africa (Fiallo-Olivé et al. 2011; Gharsallah et al. 2015; Navas-Castillo et al. 2011). The finding of ToCV in seven states of Nigeria raises the question of whether this virus could emerge, or it is already present, in other tropical countries of sub-Saharan Africa. The presence of ToCV would increase the losses already caused by viruses and other pathogens to tomato, a food and cash crop of increasing importance in this region.References:Fiallo-Olivé, E., et al. 2011. Plant Dis. 95:1592. https://doi.org/10.1094/PDIS-08-11-0631 Link, ISI, Google ScholarFortes, I. M., et al. 2012. Plant Pathol. 61:994. https://doi.org/10.1111/j.1365-3059.2011.02584.x Crossref, ISI, Google ScholarGharsallah, C., et al. 2015. Phytoparasitica 43:87. https://doi.org/10.1007/s12600-014-0423-4 Crossref, ISI, Google ScholarNavas-Castillo, J., et al. 2011. Annu. Rev. Phytopathol. 49:219. https://doi.org/10.1146/annurev-phyto-072910-095235 Crossref, ISI, Google ScholarThis work was partially supported by grants (AGL2013-48913-C2-1-R and AGL2016-75819-C2-2-R) from the Ministerio de Economía y Competitividad (MINECO, Spain), co-financed by the European Regional Development Fund (ERDF), to J. Navas-Castillo. E. Fiallo-Olivé is a recipient of a “Juan de la Cierva-Incorporación” postdoctoral contract from MINECO.DetailsFiguresLiterature CitedRelated Vol. 102, No. 1 January 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Dec 2017Published: 6 Nov 2017First Look: 12 Sep 2017Accepted: 9 Sep 2017 Page: 257 Information© 2018 The American Phytopathological SocietyCited byTomato chlorosis virus, a promiscuous virus with multiple host plants and whitefly vectors2 November 2022 | Annals of Applied Biology, Vol. 182, No. 1Bemisia tabaci (tobacco whitefly)CABI Compendium, Vol. CABI CompendiumTomato chlorosis virus (yellow leaf disorder of tomato)CABI Compendium, Vol. CABI CompendiumDot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection10 March 2021 | Tropical Plant Pathology, Vol. 46, No. 2First report of Tomato chlorosis virus infecting tomato in KenyaScientific African, Vol. 7Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges2 July 2019 | Molecular Plant Pathology, Vol. 20, No. 9Biochemical, Serological, Molecular and Natural Host Studies on Tomato Chlorosis Virus in EgyptPakistan Journal of Biological Sciences, Vol. 22, No. 2Solanum lycopersicum (Tomato)6 June 2020
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