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Morphologic study of the effect of iron on pseudocyst formation in Trichomonas vaginalis and its interaction with human epithelial cells

2017; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 112; Issue: 10 Linguagem: Inglês

10.1590/0074-02760170032

ISSN

1678-8060

Autores

Geovane Dias-Lopes, Leonardo Sabóia‐Vahia, Eliane Trindade Margotti, Nilma de Souza Fernandes, Cássia Luana de Faria Castro, Francisco Odêncio Rodrigues de Oliveira, Juliana Figueiredo Peixoto, Constança Britto, Fernando Costa e Silva Filho, Patrícia Cuervo, José Batista de Jesus,

Tópico(s)

Amoebic Infections and Treatments

Resumo

Trichomonas vaginalis is the aetiological agent of human trichomoniasis, which is one of the most prevalent sexually transmitted diseases in humans. Iron is an important element for the survival of this parasite and the colonisation of the host urogenital tract.In this study, we investigated the effects of iron on parasite proliferation in the dynamics of pseudocyst formation and morphologically characterised iron depletion-induced pseudocysts.We performed structural and ultrastructural analyses using light microscopy, scanning electron microscopy and transmission electron microscopy.It was observed that iron depletion (i) interrupts the proliferation of T. vaginalis, (ii) induces morphological changes in typical multiplicative trophozoites to spherical non-proliferative, non-motile pseudocysts, and (iii) induces the arrest of cell division at different stages of the cell cycle; (iv) iron is the fundamental element for the maintenance of typical trophozoite morphology; (v) pseudocysts induced by iron depletion are viable and reversible forms; and, finally, (vi) we demonstrated that pseudocysts induced by iron depletion are able to interact with human epithelial cells maintaining their spherical forms.Together, these data suggest that pseudocysts could be induced as a response to iron nutritional stress and could have a potential role in the transmission and infection of T. vaginalis.

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