Produção Nacional
Revisado por pares
SAPONIFICAÇÃO ASSISTIDA POR MICRO-ONDAS NA EXTRAÇÃO DE DITERPENOS EM CAFÉ ARÁBICA TORRADO
2017; Brazilian Chemical Society; Linguagem: Inglês
10.21577/0100-4042.20170116
ISSN1678-7064
AutoresMirelli Bianchin, Fábio Yamashita, Marta de Toledo Benassi,
Tópico(s)Sensory Analysis and Statistical Methods
ResumoROASTED ARABICA COFFEE.The extraction of kahweol and cafestol involves saponification reaction.This step is important for the efficiency of the extraction as the diterpenes are susceptible to structural changes during hydrolysis.The microwave-assisted saponification has been successfully used in different matrices, but there is no previous report of the use of this technique in the saponification of diterpenes.Therefore, the effect of microwave-assisted saponification on the extraction of kahweol and cafestol in roasted coffee was evaluated.A 3 2 factorial experimental design was used in order to evaluate the effect of temperature (70, 80, and 90 ºC) and reaction time (4, 8, and 12 min).The quantification of diterpenes was performed by high-performance liquid chromatography and the results were compared to with those obtained by a method, which uses thermostatic bath for saponification.Temperature and time had a significant effect (p < 0.05) on diterpenes levels.For simultaneous saponification of kahweol and cafestol, the optimal reaction conditions were 80 ºC / 12 min.The use of microwave-assisted procedure lead to lower yields of kahweol (24%) and cafestol (35%) compared to reference method.However a significant reduction of reaction time (80%) was achieved, and the procedure also has the advantage of controlled process conditions and the possibility of extended scale.
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