Produção Nacional
Revisado por pares
First Report of Colletotrichum theobromicola and C. tropicale Causing Anthracnose on Fruits of Carnauba Palm in Brazil
2017; American Phytopathological Society; Volume: 102; Issue: 1 Linguagem: Inglês
10.1094/pdis-06-17-0860-pdn
ISSN1943-7692
AutoresMaria Bruna Medeiros Araújo, Cristiano S. Lima, Francisco de Assis Câmara Rabelo Filho, Márcio Akio Ootani, Antônio Aécio de Carvalho Bezerra, J. E. Cardoso,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 102, No. 1First Report of Colletotrichum theobromicola and C. tropicale Causing Anthracnose on Fruits of Carnauba Palm in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum theobromicola and C. tropicale Causing Anthracnose on Fruits of Carnauba Palm in BrazilM. B. M. Araújo, C. S. Lima, F. de A. C. Rabelo Filho, M. A. Ootani, A. M. E. Bezerra, and J. E. CardosoM. B. M. Araújo, C. S. Lima†Corresponding author: C. S. Lima; E-mail: E-mail Address: [email protected]http://orcid.org/0000-0003-3214-5901, F. de A. C. Rabelo Filho, M. A. Ootani, A. M. E. Bezerra, and J. E. CardosoAffiliationsAuthors and Affiliations M. B. M. Araújo C. S. Lima † F. de A. C. Rabelo Filho , Departamento de Fitotecnia, Universidade Federal do Ceará, Fortaleza, Ceará 60356-001, Brazil M. A. Ootani , Embrapa Agroindústria Tropical, Fortaleza, Ceará 60511-110, Brazil A. M. E. Bezerra , Departamento de Fitotecnia, Universidade Federal do Ceará, Fortaleza, Ceará 60356-001, Brazil J. E. Cardoso , Embrapa Agroindústria Tropical, Fortaleza, Ceará 60511-110, Brazil. Published Online:9 Nov 2017https://doi.org/10.1094/PDIS-06-17-0860-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The carnauba palm (Copernicia prunifera [Mill.] H.E.Moore) is used for the production of industrial and artisanal commodities (Gomes and Nascimento 2006). Between 1990 and 2006, the northeast region produced 66,067 t of carnauba wax (Alves and Coelho 2008). In a previous report, the etiology of anthracnose on carnauba palm fruits was attributed to C. gloeosporioides (Freire and Barguil 2009). This study aimed to characterize the etiological agents of anthracnose on carnauba fruits collected in Ceará State, Brazil. Carnauba fruits at physiological maturation stage (yellowish-green color), showing severe anthracnose symptoms, were collected in Bela Cruz, Caucaia, and Paraipaba municipalities. A representative isolate to each municipality was selected for the morphological, molecular, and pathogenic characterization (UFCM 0630-Caucaia; UFCM 0631-Bela Cruz; and UFCM 0632-Paraipaba). The isolates were grown on potato dextrose agar medium at 25°C and a 12-h photoperiod and the morphological characteristics were observed after 4 days of incubation. Fungal DNA of the isolates was extracted (Murray and Thompson 1980), and fragments of the ITS rDNA (ITS), actin (ACT), β-tubulin (TUB2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and glutamine synthetase (GS) genomic regions (Prihastuti et al. 2009) were amplified and sequenced in both directions (GenBank accession nos. MF289371–85). Multiple alignments of the combined dataset of the isolates and representative sequences obtained from GenBank were phylogenetically analyzed by neighbor-joining and bootstrap with 1,000 replicates. The pathogenicity of the isolates was assessed on carnauba (n = 10, yellowish-green stage) and mango cv. Tommy Atkins (n = 3, yellowish-orange stage) fruits, and on susceptible cashew plantlets (clone BRS 265) (n = 3, 120 days old). Wounded fruits were inoculated with 3-mm agar plugs containing mycelium of the isolates, while control treatments had only the agar. Spore suspensions (2 × 106 conidia ml−1) were sprayed on young leaves of the cashew plantlets, while only water was sprayed on control plants. The UFCM 0630 and UFCM 0632 isolates formed pale olive-gray aerial mycelium and olive-gray pigment in the back of the colonies. Conidia were cylindrical, 5.4 to 13.8 × 1.5 to 4.7 (mean = 9.0 × 2.9, n = 50) µm. Appressoria were subglobose to elliptical, 2.8 to 5.8 × 2.4 to 3.1 (mean = 4.8 × 2.8, n = 10) μm. The UFCM 0631 isolate formed dark gray aerial mycelium and dark greenish-gray pigment in the back of the colonies. Conidia were cylindrical, 13.0 to 17.5 × 2.3 to 5.5 (mean = 12.1 × 3.7, n = 50) µm. Appressoria were subglobose to clavate, 6.0 to 9.5 × 4.2 to 7.6 (mean = 7.8 × 5.9, n = 10) μm. In the phylogenetic tree, the isolates of C. prunifera grouped in two clades of the C. gloeosporioides species complex. UFCM 0630 and UFCM 0632 isolates grouped in C. tropicale and UFCM 0631 grouped in C. theobromicola with 100% bootstrap support to both clades. Koch's postulates were completed on inoculated carnauba fruits to all three isolates representing C. tropicale and C. theobromicola. Only UFCM 0630 and UFCM 0632 isolates were pathogenic to mango fruits and cashew plantlets. This study shows evidence that carnauba palm may serve as an alternative host of C. tropicale and inoculum source for mango and cashew anthracnose in orchards established near to natural areas of carnauba. To our knowledge, this is the first report of C. tropicale and C. theobromicola causing anthracnose on carnauba in Brazil.References:Alves, M. O., and Coelho, J. D. 2008. Banco Nordeste Brasil 20:214. Google ScholarFreire, F. das C. O., and Barguil, B. M. 2009. Summa Phytopathol. 35:68. https://doi.org/10.1590/S0100-54052009000100013 Crossref, Google ScholarGomes, J. M. A., and Nascimento, W. L. 2006. Page 190 in: Cadeia Produtiva da Cera de Carnaúba: Diagnóstico e Cenários. EDUFPI, Teresina, Piauí, Brazil. Google ScholarMurray, M. G., and Thompson, W. F. 1980. Nucleic Acids Res. 8:4321. https://doi.org/10.1093/nar/8.19.4321 Crossref, ISI, Google ScholarPrihastuti, H., et al. 2009. Fungal Divers. 39:89. ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 1 January 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Dec 2017Published: 9 Nov 2017First Look: 13 Sep 2017Accepted: 9 Sep 2017 Page: 244 Information© 2018 The American Phytopathological SocietyCited byTwo additional Colletotrichum species causing leaf spot of rambutan ( Nephelium lappaceum )16 March 2023 | Archives of Phytopathology and Plant Protection, Vol. 102Pest categorisation of Colletotrichum aenigma, C. alienum, C. perseae, C. siamense and C. theobromicolaEFSA Journal, Vol. 20, No. 8Colletotrichum tropicaleCABI Compendium, Vol. CABI CompendiumColletotrichum theobromicolaCABI Compendium, Vol. CABI CompendiumA serious shoot and leaf disease caused by Colletotrichum theobromicola discovered on eucalypts in South Africa22 March 2022 | Southern Forests: a Journal of Forest Science, Vol. 84, No. 1Identification and characterisation of Colletotrichum fructicola, C. tropicale and C. theobromicola causing mango anthracnose in the Philippines19 August 2021 | Archives of Phytopathology and Plant Protection, Vol. 54, No. 19-20Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1First Report of Colletotrichum tropicale Causing Anthracnose on Pomegranate in BrazilJ. R. A. Silva-Cabral, L. R. L. Batista, J. F. de O. Costa, M. M. de M. Ferro, S. J. C. Silva, G. S. de A. Lima, and I. P. Assunção17 January 2019 | Plant Disease, Vol. 103, No. 3Diversity of pathogenic and endophytic Colletotrichum isolates from Licania tomentosa in Brazil30 May 2018 | Forest Pathology, Vol. 48, No. 6
Referência(s)