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Pinus pinaster Knot: A Source of Polyphenols against Plasmopara viticola

2017; American Chemical Society; Volume: 65; Issue: 40 Linguagem: Inglês

10.1021/acs.jafc.7b04129

1520-5118

Julien Gabaston, Tristan Richard, Stéphanie Cluzet, Antonio Palos Pinto, Marie‐Cécile Dufour, Marie‐France Corio‐Costet, Jean‐Michel Mérillon,

Forest Insect Ecology and Management

Pine knot extract from Pinus pinaster byproducts was characterized by UHPLC-DAD-MS and NMR. Fourteen polyphenols divided into four classes were identified as follows: lignans (nortrachelogenin, pinoresinol, matairesinol, isolariciresinol, secoisolariciresinol), flavonoids (pinocembrin, pinobanksin, dihydrokaempferol, taxifolin), stilbenes (pinosylvin, pinosylvin monomethyl ether, pterostilbene), and phenolic acids (caffeic acid, ferulic acid). The antifungal potential of pine knot extract, as well as the main compounds, was tested in vitro against Plasmopara viticola. The ethanolic extract showed a strong antimildew activity. In addition, pinosylvins and pinocembrin demonstrated significant inhibition of zoospore mobility and mildew development. These findings strongly suggest that pine knot is a potential biomass that could be used as a natural antifungal product.