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Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin

2017; Elsevier BV; Volume: 493; Issue: 2 Linguagem: Inglês

10.1016/j.bbrc.2017.09.056

1090-2104

Yoon‐Jin Kim, Sae Mi Yoo, Hwan Hee Park, Hye Jin Lim, Yu-Lee Kim, Seunghee Lee, Kwang–Won Seo, Kyung‐Sun Kang,

Advancements in Transdermal Drug Delivery

Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) play an important role in cutaneous wound healing, and recent studies suggested that MSC-derived exosomes activate several signaling pathways, which are conducive in wound healing and cell growth. In this study, we investigated the roles of exosomes that are derived from USC-CM (USC-CM Exos) in cutaneous collagen synthesis and permeation. We found that USC-CM has various growth factors associated with skin rejuvenation. Our in vitro results showed that USC-CM Exos integrate in Human Dermal Fibroblasts (HDFs) and consequently promote cell migration and collagen synthesis of HDFs. Moreover, we evaluated skin permeation of USC-CM Exos by using human skin tissues. Results showed that Exo-Green labeled USC-CM Exos approached the outermost layer of the epidermis after 3 h and gradually approached the epidermis after 18 h. Moreover, increased expressions of Collagen I and Elastin were found after 3 days of treatment on human skin. The results showed that USC-CM Exos is absorbed into human skin, it promotes Collagen I and Elastin synthesis in the skin, which are essential to skin rejuvenation and shows the potential of USC-CM integration with the cosmetics or therapeutics.