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Vibrio species involved in seafood-borne outbreaks ( Vibrio cholerae, V. parahaemolyticus and V. vulnificus ): Review of microbiological versus recent molecular detection methods in seafood products

2017; Taylor & Francis; Volume: 59; Issue: 4 Linguagem: Inglês

10.1080/10408398.2017.1384715

1549-7852

Maryse Bonnin-Jusserand, Stéphanie Copin, Cédric Le Bris, Thomas Brauge, Mélanie Gay, Anne Brisabois, Thierry Grard, Graziella Midelet,

Aquaculture disease management and microbiota

Seafood products are widely consumed all around the world and play a significant role on the economic market. Bacteria of the Vibrio genus can contaminate seafood and thus pose a risk to human health. Three main Vibrio species, V. cholerae, V. parahaemolyticus and V. vulnificus, are potentially pathogenic to humans. These species are responsible for a dramatic increase of seafood-borne infections worldwide. Hence, early detection of total and pathogenic Vibrio is needed and should rely on quick and effective methods. This review aims to present the standard methods FDA-BAM, ISO/TS 21872-1:2007 and TS 21872-2:2007 and compare them to recent molecular biology methods including endpoint PCR, quantitative real-time PCR (qPCR) and PCR-derived methods with a focus on LAMP (loop-mediated isothermal amplification). The available methods presented here are dedicated to the detection and identification of the Vibrio species of interest in seafood.