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Impact of cellular health conditions on the protein folding state in mammalian cells

2017; Royal Society of Chemistry; Volume: 53; Issue: 81 Linguagem: Inglês

10.1039/c7cc06004a

1364-548X

Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, T. Kigawa,

Endoplasmic Reticulum Stress and Disease

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.