Portal de Periódicos da CAPES

Longitudinal evaluation of myocardial glucose metabolism and contractile function in obese type 2 diabetic db/db mice using small-animal dynamic 18F-FDG PET and echocardiography

2017; Impact Journals LLC; Volume: 8; Issue: 50 Linguagem: Inglês

10.18632/oncotarget.21202

1949-2553

Kuan‐Yin Ko, Yen‐Wen Wu, Cheng‐Wei Liu, Mei‐Fang Cheng, Ruoh‐Fang Yen, Wei‐Shiung Yang,

Cardiac Imaging and Diagnostics

// Kuan-Yin Ko 1, 2,8 , Yen-Wen Wu 2, 3, 4, 5, 6 , Cheng-Wei Liu 5, 7, 8 , Mei-Fang Cheng 2, 9 , Ruoh-Fang Yen 2 and Wei-Shiung Yang 3, 8, 10, 11 1 Department of Nuclear Medicine, National Taiwan University Hospital, Yunlin Branch, Yunlin County, Taiwan 2 Department of Nuclear Medicine, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan 3 Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan 4 National Yang-Ming University School of Medicine, Taipei, Taiwan 5 Cardiology Division of Cardiovascular Medical Center, Far Eastern Memorial Hospital, New Taipei City, Taiwan 6 Department of Nuclear Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan 7 Department of Internal Medicine, Tri-Service General Hospital, Songshan Branch, National Defense Medical Center, Taipei, Taiwan 8 Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan 9 Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University, Taipei, Taiwan 10 Department of Medicine and Graduate Institute of Medical Genomics & Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan 11 R & D Branch Office, College of Medicine, National Taiwan University, Taipei, Taiwan Correspondence to: Yen-Wen Wu, email: wuyw0502@gmail.com Keywords: type 2 diabetes mellitus (DM), db/db mice, small animal imaging, positron emission tomography (PET), 18 F-fluorodeoxyglucose ( 18 F-FDG) Received: June 22, 2017 Accepted: August 17, 2017 Published: September 23, 2017 ABSTRACT The aim was to evaluate sequential changes of myocardial glucose utilization and LV systolic function in db/db mice. Eight db/db and eight wild-type mice underwent plasma substrate analysis and dynamic 18 F-FDG PET at week 8 (W8), W10, W12, W14, and W16. 18 F-FDG uptake constant Ki and the rate of myocardial glucose uptake (MRGlu) were derived via Patlak graphic analysis. Another 8 db/db and 8 wild-type mice received echocardiography at W8, W12, and W16 and LV structure and function were measured. The db/db mice showed increased weights and glucose levels as they aged. The index of homeostasis model assessment-estimated insulin resistance, insulin, and free fatty acid concentrations were higher in db/db mice compared with wild-type. MRGlu of db/db mice across all time points was markedly higher than that of wild-type. An age-dependent elevation of MRGlu was observed in db/db mice. Ki and MRGlu of db/db mice showed negative correlation with triglyceride levels. When two groups were pooled together, Ki and MRGlu were significantly proportional to glucose levels. No significant difference in LV structure and function was noted between db/db and control mice. In conclusion, we demonstrated altered myocardial glucose utilization preceding the onset of LV systolic dysfunction in db/db mice.