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First Report of Soybean mosaic virus and Soybean yellow mottle mosaic virus in Vigna angularis

2017; American Phytopathological Society; Volume: 102; Issue: 3 Linguagem: Inglês

10.1094/pdis-08-17-1284-pdn

1943-7692

Young-Sik Yoon, Seungmo Lim, Yunwoo Jang, Byung–Sup Kim, D. H. Bae, Rameswor Maharjan, H. Yi, Seon-Hwa Bae, Y.-H. Lee, B. C. Lee, Jae Sun Moon, C.-Y. Park, Su-Heon Lee,

Plant Pathogenic Bacteria Studies

HomePlant DiseaseVol. 102, No. 3First Report of Soybean mosaic virus and Soybean yellow mottle mosaic virus in Vigna angularis PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Soybean mosaic virus and Soybean yellow mottle mosaic virus in Vigna angularisY. Yoon, S. Lim, Y.-W. Jang, B.-S. Kim, D. H. Bae, R. Maharjan, H. Yi, S. Bae, Y.-H. Lee, B. C. Lee, J. S. Moon, C.-Y. Park, and S.-H. LeeY. Yoon, S. Lim, Y.-W. Jang, B.-S. Kim, D. H. Bae, R. Maharjan, H. Yi, S. Bae, Y.-H. Lee, B. C. Lee, J. S. Moon, C.-Y. Park, and S.-H. Lee†Corresponding author: S.-H. Lee; E-mail: E-mail Address: [email protected]AffiliationsAuthors and Affiliations Y. Yoon S. Lim Y.-W. Jang B.-S. Kim D. H. Bae R. Maharjan H. Yi S. Bae , Crop Production Technology Research Division, National Institute of Crop Science, RDA, Miryang 50424, Republic of Korea Y.-H. Lee , Planning and Coordination Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea B. C. Lee , Crop Foundation Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea J. S. Moon , Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea C.-Y. Park S.-H. Lee † , School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea. Published Online:20 Dec 2017https://doi.org/10.1094/PDIS-08-17-1284-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In 2015 and 2016, 203 samples of adzuki bean (Vigna angularis) exhibiting viral disease-like symptoms, such as mosaic, mottle, yellowing, dwarfing, and leaf rolling, were collected from 18 cities and counties in Korea. Total RNA was prepared from a pooled sample using an easy-spin Total RNA Extraction kit (iNtRON Biotechnology, Seongnam, Korea) and used for high-throughput paired-end RNA sequencing. Raw data analysis was conducted with an Illumina HiSeq 2500 system at the Theragen Etex Bio Institute (Suwon, Korea) and SG-VIPdb at SeqGenesis (Daejeon, Korea), respectively, as previously described (Lim et al. 2015). As expected, 239 and 10 contigs related to Bean common mosaic virus and Cucumber mosaic virus, which have been previously reported in adzuki bean, were obtained from the adzuki bean transcriptome, respectively. However, in addition to this, 143 and 8 contigs were attributed to two soybean viruses, Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV), respectively. To confirm the presence of SMV and SYMMV, reverse transcription (RT) polymerase chain reaction (PCR) was performed using SuPrimeScript RT-PCR Premix (GeNet Bio, Daejeon, Korea) with an SMV-specific primer set, SM-N40 5′-CATATCAGTTTGTTGGGCA-3′ and SM-C20 5′-TGCCTATACCCTCAACAT-3′ (expected size of 492 bp), and an SYMMV-specific primer set, SYMM-F34 5′-CAACCCTCAGCCACATTCAACTAT-3′ and SYMM-R40 5′-TCTAACCACCCCACCCGAAGGATT-3′ (expected size of 597 bp), respectively (Lim et al. 2014). Of the 203 samples, 33 and 57 samples were found infected with SMV and SYMMV, respectively, and 17 samples were found infected with both SMV and SYMMV. RT-PCR-amplified fragments from the selected samples were cloned into the RBC T&A cloning vector (RBC Bioscience, Taipei, Taiwan) and subsequently sequenced (Macrogen, Daejeon, Korea). One 9,584-nt complete genome sequence of SMV from V. angularis was determined (isolate CC2515; GenBank accession no. KY986929). The BLASTn search showed that it had the highest nucleotide sequence identity (99%) with SMV strain G4 from G. max in Korea (GenBank accession no. FJ640979). One 1,065-nt coat protein gene sequence of SYMMV from V. angularis was also determined (isolate CC2245; GenBank accession no. MF716520), and the BLASTn search showed that it shared the highest nucleotide sequence identity (98%) with SYMMV strain Gimhae from G. soja in Korea (GenBank accession no. FJ494834). To the best of our knowledge, this is the first report of SMV and SYMMV infecting V. angularis.References:Lim, S., et al. 2014. Plant Dis. 98:1285. https://doi.org/10.1094/PDIS-04-14-0356-PDN Link, ISI, Google ScholarLim, S., et al. 2015. Arch. Virol. 160:3153. https://doi.org/10.1007/s00705-015-2616-7 Crossref, ISI, Google ScholarY. Yoon and S. Lim contributed equally to this study. The authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.This research was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (project no. PJ01112801), Rural Development Administration, Republic of Korea.DetailsFiguresLiterature CitedRelated Vol. 102, No. 3 March 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 19 Feb 2018Published: 20 Dec 2017First Look: 3 Oct 2017Accepted: 30 Sep 2017 Pages: 689-689 Information© 2018 The American Phytopathological SocietyCited byCucumber mosaic virus (cucumber mosaic)CABI Compendium, Vol. CABI CompendiumSoybean mosaic virus (soybean mosaic)CABI Compendium, Vol. CABI CompendiumSoybean yellow mottle mosaic virusCABI Compendium, Vol. CABI CompendiumBean common mosaic virus (common mosaic of beans)CABI Compendium, Vol. CABI CompendiumAn Overview on Soybean Mosaic Virus and Its Management6 May 2022Weeds as Reservoirs of Viruses in Agrobiocenoses of Legumes in Ukraine30 November 2020 | Mikrobiolohichnyi Zhurnal, Vol. 82, No. 6Synthesis of Full-Length cDNA Infectious Clones of Soybean Mosaic Virus and Functional Identification of a Key Amino Acid in the Silencing Suppressor Hc-Pro13 August 2020 | Viruses, Vol. 12, No. 8Genetic evolutionary analysis of soybean mosaic virus populations from three geographic locations in China based on the P1 and CP genes11 February 2019 | Archives of Virology, Vol. 164, No. 4