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Methods for genetic transformation of filamentous fungi

2017; BioMed Central; Volume: 16; Issue: 1 Linguagem: Inglês

10.1186/s12934-017-0785-7

1475-2859

Dandan Li, Yu Tang, Jun Lin, Weiwen Cai,

CRISPR and Genetic Engineering

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.