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Studies for Obtaining the Protocorms and Plantlets in Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum under in vitro Conditions

2017; Volume: 3; Issue: 3 Linguagem: Inglês

10.20431/2454-9487.0303005

2454-9487

Cevdet Gümüş, Şeküre Şebnem Ellialtıoğlu, S. F. Eman,

GABA and Rice Research

Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum protocorms and plantlets were obtained via asymbiotic germination under in vitro conditions.For the germination of seeds, 4 basic nutrient media consisting of half-strength MS (½ MS), full-strength MS, Van Waes DeBergh (VW and DB), and Knudson C. The petri dishes contained orchid seeds and the nutrient medium semi-solidified with agar (0.6%).cultures were incubated in darkness for the first 3 months and thereafter transferred to a light/dark photoperiod of 16/8 hours.At the end of the third month, protocorm rates were identified.They are subcultured every 4 weeks and ½ MS medium was used as a transfer medium.The highest germination rate, protocorm rate and plant growth rate were obtained from Knudson C medium.