Portal de Periódicos da CAPES

A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata

2017; Elsevier BV; Volume: 79; Linguagem: Inglês

10.1016/j.dci.2017.10.016

1879-0089

Leah M. Williams, Lauren E. Fuess, Joseph J. Brennan, Katelyn M. Mansfield, Erick Salas-Rodriguez, Julianne Welsh, Jake Awtry, Sarah Banic, Cecilia Chacko, Aarthia Chezian, Donovan Dowers, Felicia Estrada, Yu‐Hsuan Hsieh, Jiawen Kang, Wanwen Li, Zoe X. Malchiodi, John Malinowski, Sean J. Matuszak, Thomas McTigue, David Mueller, Brian Nguyen, Michelle Nguyen, Phuong Nguyen, Sinead Nguyen, Ndidi Njoku, Khusbu Patel, William R. Pellegrini, Tessa Pliakas, Deena Qadir, Emma Ryan, Alex Schiffer, Amber Thiel, Sarah A. Yunes, Kathryn E. Spilios, Jorge H. Pinzón, Laura D. Mydlarz, Thomas D. Gilmore,

Immune Response and Inflammation

Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo. These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways.