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Influence of the addition of microsphere load amoxicillin in the physical, chemical and biological properties of an experimental endodontic sealer

2017; Elsevier BV; Volume: 68; Linguagem: Inglês

10.1016/j.jdent.2017.10.010

1879-176X

Nélio Bairros Dornelles, Fabrício Mezzomo Collares, Bruna Genari, Gabriela de Souza Balbinot, Susana Maria Werner Samuel, Rodrigo Alex Arthur, Fernanda Visioli, Sı́lvia Stanisçuaski Guterres, Vicente Castelo Branco Leitune,

Dental Anxiety and Anesthesia Techniques

To develop an endodontic sealer with amoxicillin-loaded microsphere and to evaluate its properties. Experimental sealer was obtained by mixing 70 wt% UDMA, 15 wt% GDMA and 15 wt% BISEMA. In addition, CQ, DHEPT and PB were incorporated at 1 mol% and 0.01 wt% BHT and 10 wt% YbF3. Microspheres were produced by drying in spray dryer. Two experimental groups (10 and 15 wt% of microspheres) and one control group (without microspheres) were formulated. The sealers were evaluated for the degree of conversion (n = 3), degradation in solvent (n = 3), drug release profile (n = 3), antimicrobial activity (n = 3), flow (n = 3), film thickness (n = 3) and cytotoxicity (n = 3). Data were analyzed by ANOVA and Tukey with significance level of 5%. Microespheres presented a mean size of 2.664 μm. Immediate degree of conversion ranged from 51.73% to 55.13% and the 24 h degree of conversion ranged from 60.79% to 73.80%. Upon solvent degradation 54.44% and 56.21% reduction in hardness were observed for 10% and 15% concentrations respectivelly. The drug release profile showed an average release of 73.76% of the drug in 96 h. Significant reduction in antimicrobial activity was observed for 10% concentration after 24 h, 48 h and 96 h compared to control. The flow and film thickness showed values in accordance to the ISO 6876. Cytotoxicity showed high cellular viability. The addition of up to 10% of microspheres containing amoxicillin presented antimicrobial activity and did not alter the properties of the experimental endodontic cement. Amoxicillin microspheres with its antimicrobial activity in root canal sealers could reduce reintervations in endodontics when persistent bacteria or reinfection takes place in root canal system.