Produção Nacional
Revisado por pares
Staphylococcus aureus in Some Brazilian Dairy Industries: Changes of Contamination and Diversity
2017; Frontiers Media; Volume: 8; Linguagem: Inglês
10.3389/fmicb.2017.02049
ISSN1664-302X
AutoresKaren K. Dittmann, Luíza Toubas Chaul, Sarah Hwa In Lee, Carlos Humberto Corassin, Carlos Augusto Fernandes de Oliveira, Elaine Cristina Pereira De Martinis, Virgínia Farias Alves, Lone Gram, Virginie Oxaran,
Tópico(s)Bacterial biofilms and quorum sensing
ResumoStaphylococcus aureus, a major food-poisoning pathogen, is a common contaminant in dairy industries worldwide, including in Brazil. We determined the occurrence of S. aureus in five dairies in Brazil over eight months. Of 421 samples, thirty-one (7.4%) were positive for S. aureus and prevalence varied from 0 to 63.3% between dairies. Sixty-six isolates from the 31 samples were typed by Multi-Locus Sequence Typing to determine if these isolates were persistent or continuously reintroduced. Seven known Sequence Types (ST), ST1, ST5, ST30, ST97, ST126, ST188 and ST398, and four new ST were identified, ST3531, ST3540, ST3562 and ST3534. Clonal Complex (CC) 1 (including the three new ST), known as an epidemic clone, was the dominant CC. However, there were no indications of persistence of particular ST. The resistance towards eleven antibiotic compounds was assessed. Twelve profiles were generated with 75.8% of strains being sensitive to all antibiotic classes and no Methicillin-resistant S. aureus (MRSA) strains were found. The enterotoxin-encoding genes involved in food-poisoning, e.g. sea, sed, see and seg were targeted by PCR. The two toxin-encoding genes, sed and see, were not detected. Only three strains (4.5%) harbored seg and two of these also harbored sea. Despite the isolates being Methicillin-sensitive S. aureus (MSSA), the presence of CC1 clones in the processing environment, including some harboring enterotoxin encoding genes, is of concern and hygiene must have high priority to reduce contamination.
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