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A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

2017; Taylor & Francis; Volume: 32; Issue: 24 Linguagem: Inglês

10.1080/14786419.2017.1389932

1478-6427

Emmanuel Viana Pontual, Divar F. Pires-Neto, Karina Fraige, Taciana Mirely Maciel Higino, Belany Emanuele Alves de Carvalho, Neyla Maria Pereira Alves, Thâmarah A Lima, Russolina B. Zingali, Luana Cassandra Breitenbach Barroso Coelho, Vanderlan da Silva Bolzani, Regina Célia Bressan Queiroz de Figueiredo, Thiago Henrique Napoleão, Patrícia Maria Guedes Paiva,

Traditional and Medicinal Uses of Annonaceae

In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 ± 6.62 and 41.20 ± 4.28 μg/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract.