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First Report of Anthracnose Caused by Colletotrichum sichuanensis on Phaseolus lunatus in Brazil
2017; American Phytopathological Society; Volume: 102; Issue: 3 Linguagem: Inglês
10.1094/pdis-07-17-0963-pdn
ISSN1943-7692
AutoresGerusa R. S. Cavalcante, Willie Anderson dos Santos Vieira, Sami Jorge Michereff, Beatriz Meireles Barguil, Vinson P. Doyle, Marcos Paz Saraiva Câmara,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 102, No. 3First Report of Anthracnose Caused by Colletotrichum sichuanensis on Phaseolus lunatus in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Anthracnose Caused by Colletotrichum sichuanensis on Phaseolus lunatus in BrazilG. R. S. Cavalcante, W. A. S. Vieira, S. J. Michereff, B. M. Barguil, V. P. Doyle, and M. P. S. CâmaraG. R. S. CavalcanteSearch for more papers by this author, W. A. S. VieiraSearch for more papers by this author, S. J. MichereffSearch for more papers by this author, B. M. BarguilSearch for more papers by this author, V. P. DoyleSearch for more papers by this author, and M. P. S. Câmara†Corresponding author: M. P. S. Câmara; E-mail: E-mail Address: marcos.camara@ufrpe.brSearch for more papers by this authorAffiliationsAuthors and Affiliations G. R. S. Cavalcante W. A. S. Vieira S. J. Michereff , Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil B. M. Barguil , Departamento de Biologia, Universidade Estadual do Piauí, Teresina, 64078-213, Piauí, Brazil V. P. Doyle , Department of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, Baton Rouge, 70803 M. P. S. Câmara † , Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil. Published Online:15 Jan 2018https://doi.org/10.1094/PDIS-07-17-0963-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The lima bean (Phaseolus lunatus) is of cultural importance in the Northeast region of Brazil as a source of income and food for the population. Among the diseases impacting lima bean production, anthracnose caused by the genus Colletotrichum is among the most prominent. In 2015, lima bean leaves with typical symptoms of anthracnose were collected in five states of northeastern Brazil, including Pernambuco, Paraíba, Piauí, Ceará, and Alagoas. The symptoms are characterized by reddish spots on the leaf blade and along the primary and secondary veins, resulting in wrinkled leaves. On pods and stems, the lesions are depressed and reddish with developmental acervuli. Small pieces of leaf tissue from the periphery of the lesions were surface disinfested (1 min in 70% alcohol, 2 min in 1% NaOCl, and rinsed with sterile water), plated on potato dextrose agar (PDA), and incubated at 25°C for 7 days. Colonies that were morphologically similar to Colletotrichum spp. were transferred to PDA and preserved. In order to identify the Colletotrichum species, partial sequences of glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacer, actin, and β-tubulin regions were generated for five isolates and deposited in GenBank (accession nos. MF279901 to MF279921). Sequences representing ex-type and authentic isolates of species closely related based on the BLAST results were aligned with the aforementioned sequences, and a maximum likelihood phylogenetic analysis was used to confirm the identity. The resulting maximum likelihood topology placed the isolates from lima bean in the same clade as Colletotrichum sichuanensis with 93% bootstrap support. Morphological and cultural characteristics were examined on PDA at 25°C under continuous fluorescent light for 7 days. Isolates presented white to grayish mycelium, with dark brown to dark gray reverse. Conidia on PDA were hyaline, cylindrical, aseptate, 7.8 to 11 μm in length, and 2.0 to 4.0 μm in width (n = 30). The conidial measurements were smaller than the original description of C. sichuanensis (Liu et al. 2016), indicating morphological characteristics are variable for the species, as observed in other Colletotrichum species (Cai et al. 2009). However, the phylogenetic analysis confidently assigned the isolates to the species C. sichuanensis. To confirm pathogenicity, five replicate 40-day-old lima bean plants, without any symptom of anthracnose, were sprayed with a conidial suspension (105 conidia/ml) from each isolate. Plants inoculated with sterilized distilled water were used as a control. The plants were placed in a humid chamber for 36 h at 25°C with a photoperiod of 12 h. Typical symptoms of anthracnose were observed 5 days after inoculation, and no symptoms were observed on control plants. The pathogen was reisolated from the lesions, fulfilling Koch’s postulates. C. sichuanensis was originally described as causing anthracnose in peppers in China’s Sichuan Province. To our knowledge, this is the first report of C. sichuanensis in Brazil and causing anthracnose in lima bean.References:Cai, F., et al. 2009. Fungal Divers. 39:183. ISI, Google ScholarLiu, F., et al. 2016. Sci. Rep. 6:32761. https://doi.org/10.1038/srep32761 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 3 March 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 19 Feb 2018Published: 15 Jan 2018First Look: 7 Nov 2017Accepted: 6 Nov 2017 Pages: 680-680 Information© 2018 The American Phytopathological SocietyCited byColletotrichum species associated with sugarcane red rot in BrazilFungal Biology, Vol. 126, No. 4Colletotrichum sichuanenseCABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Lima beanFirst Report of Colletotrichum musicola Causing Soybean Anthracnose in BrazilT. R. Boufleur, R. R. L. Castro, F. Rogério, M. Ciampi-Guillardi, R. Baroncelli, and N. S. Massola Júnior17 April 2020 | Plant Disease, Vol. 104, No. 6Diversity, Prevalence, and Virulence of Colletotrichum Species Associated with Lima Bean in BrazilGerusa R. S. Cavalcante, Beatriz M. Barguil, Willie A. S. Vieira, Waléria G. Lima, Sami J. Michereff, Vinson P. Doyle, and Marcos P. S. Câmara23 May 2019 | Plant Disease, Vol. 103, No. 8The Colletotrichum dracaenophilum , C. magnum and C. orchidearum species complexesStudies in Mycology, Vol. 92, No. 1
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