Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Influence of growth regulators on distribution of trichomes and the production of volatiles in micropropagated plants of Plectranthus ornatus

2017; Springer Science+Business Media; Volume: 27; Issue: 6 Linguagem: Inglês

10.1016/j.bjp.2017.10.001

1981-528X

Helna Passinho-Soares, Juceni P. David, José Raniere Ferreira de Santana, Jorge M. David, Frederico de Medeiros Rodrigues, Paulo Roberto Ribeiro Mesquita, Fábio Santos de Oliveira, Moema Cortizo Bellintani,

Plant and animal studies

The profile of volatile organic compounds, the glandular and non-glandular trichomes of Plectranthus ornatus, obtained by in vitro cultivation, was evaluated in plants grown in Murashide and Skoog medium supplemented with benylaminopurine at 4.5, 9.0, and 18.0 μM + naphthaleneacetic acid at 5.37 μM, kinetin at 4.7, 9.3 and 18.5 μM + naphthaleneacetic acid (5.37 μM) or Murashide and Skoog 0 medium (as a control). Scanning Electron Microscopy was performed on samples of the third leaf node of the 90 days old plants obtained from treatment with 4.5 or 9.0 μM benylaminopurine, and 4.7 or 9.3 μM kinetin. Headspace Solid Phase Micro-Extraction of the 30, 60 and 90 days old in vitro plants permitted to determinate by GC/MS the composition comprised of 62 compounds. The data were analyzed using Principal Component Analysis and Hierarchical Clustering Analysis and, the major constituents of these oils after treatment and aging were monoterpenes and sesquiterpenes. Morphoanatomical analysis of trichomes, by Scanning Electron Microscopy, enabled the identification of non-glandular trichomes and four types of glandular trichomes, which comprised capitate and peltate glandular trichomes that were distributed on both sides of the leaf. We observed that the regulators influenced qualitative and quantitative profiles of the volatile organic compounds and the number and distribution of hairs on the leaf surface.