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Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

2017; Nature Portfolio; Volume: 8; Issue: 1 Linguagem: Inglês

10.1038/s41467-017-01695-x

2041-1723

Jiazhang Lian, Mohammad HamediRad, Sumeng Hu, Huimin Zhao,

RNA and protein synthesis mechanisms

Abstract Designing an optimal microbial cell factory often requires overexpression, knock-down, and knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is often carried out sequentially and with low throughput. Here, we report a combinatorial metabolic engineering strategy based on an orthogonal tri-functional CRISPR system that combines transcriptional activation, transcriptional interference, and gene deletion (CRISPR-AID) in the yeast Saccharomyces cerevisiae . This strategy enables perturbation of the metabolic and regulatory networks in a modular, parallel, and high-throughput manner. We demonstrate the application of CRISPR-AID not only to increase the production of β-carotene by 3-fold in a single step, but also to achieve 2.5-fold improvement in the display of an endoglucanase on the yeast surface by optimizing multiple metabolic engineering targets in a combinatorial manner.