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First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C
2017; American Phytopathological Society; Volume: 102; Issue: 5 Linguagem: Inglês
10.1094/pdis-07-17-1000-pdn
ISSN1943-7692
AutoresMaria Andréia Nunes, Jeferson Luiz de Carvalho Mineiro, Luana Aparecida Rogerio, Leticia Maria Ferreira, Aline Daniele Tassi, Valdenice Moreira Novelli, E. W. Kitajima, Juliana Freitas‐Astúa,
Tópico(s)Nematode management and characterization studies
ResumoHomePlant DiseaseVol. 102, No. 5First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus CM. A. Nunes, J. L. de Carvalho Mineiro, L. A. Rogerio, L. M. Ferreira, A. Tassi, V. M. Novelli, E. W. Kitajima, and J. Freitas-AstúaM. A. Nunes†Corresponding author: M. A. Nunes; E-mail: E-mail Address: [email protected], J. L. de Carvalho Mineiro, L. A. Rogerio, L. M. Ferreira, A. Tassi, V. M. Novelli, E. W. Kitajima, and J. Freitas-AstúaAffiliationsAuthors and Affiliations M. A. Nunes † , CCSM-IAC, CP 4, 13490-970 J. L. de Carvalho Mineiro , Instituto Biológico de Campinas L. A. Rogerio L. M. Ferreira , CCSM-IAC, CP 4, 13490-970 A. Tassi , Escola Superior de Agricultura "Luiz de Queiroz"/USP, Piracicaba, SP, Brazil V. M. Novelli , CCSM-IAC, CP 4, 13490-970, Cordeirópolis, SP E. W. Kitajima , Escola Superior de Agricultura "Luiz de Queiroz"/USP, Piracicaba, SP, Brazil J. Freitas-Astúa , Embrapa Cassava and Fruits, Brazil, and Instituto Biológico, SP, Brazil. Published Online:8 Mar 2018https://doi.org/10.1094/PDIS-07-17-1000-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Coffee ringspot virus (CoRSV) and Citrus leprosis virus C (CiLV-C) are two emergent pathogens that can cause significant losses to coffee and citrus, respectively. Recently, it was verified that Brevipalpus phoenicis, originally reported as vector of these viruses, is indeed a complex group reclassified into eight mite species (Beard et al. 2015). Among those, B. papayensis Baker (Acari: Tenuipalpidae) occurs in citrus, but seems to be prevalent in coffee plantations. However, it is still unclear the relationship (if any) of B. papayensis with CiLV-C or CoRSV. In order to determine whether or not this mite is capable of transmitting these viruses, one single female specimen was collected from Coffea arabica cv. Catuai in Atibaia, SP-Brazil, and multiplied in laboratory as an isoline. Specimens were mounted in Hoyer's medium and morphologically confirmed by phase contrast microscopy as B. papayensis, according to the classification of Beard et al. (2015). This isoline population was reared onto healthy coffee leaves, in 25 ± 1°C, 14 h light/10 h dark and 60 ± 10% humidity. RT-PCR assays using primers for the detection of each of the viruses were performed in order to confirm the virus-free colony status. B. papayensis mites were transferred to either coffee leaves exhibiting ringspots or sweet orange fruits symptomatic for leprosis, which served as sources of inocula for 7 days. Viral acquisition was confirmed by RT-PCR of viruliferous mites using specific primers for the detection of CoRSV (Kitajima et al. 2011) or CiLV-C (Locali et al. 2003), according to Kubo et al. (2011). After the acquisition period, 60 mites from each source of inoculum were transferred to 20 Arabidopsis thaliana plants (6 mites per plant), a plant species that can host both. Fifteen days after infestation, typical pinpoint lesions were observed and virus presence was confirmed by RT-PCR in 60% of the CiLV-C- and 30% of the CoRSV-inoculated plants. Additionally, common bean plants (Phaseolus vulgaris cv. Una) were infested with 15 B. papayensis mites from the isoline population reared onto CiLV-C inoculum (one mite per leaf, isolated by entomological glue). Perhaps due to the low efficiency in single mite transmission, only 27% of the leaves became symptomatic. The presence of CiLV-C was confirmed by RT-PCR and sequencing (98% of similarity to GenBank accession no. KP3367461). Fifteen females were collected directly from coffee leaves with CoRSV symptoms in Cordeirópolis, SP-Brazil, and transferred to isolated leaves of common bean plants. Symptoms of necrotic local lesions appeared 10 days after inoculation in 50% of the plants. The presence of CoRSV was confirmed by RT-PCR in both coffee and bean leaves. Amplicons obtained from the latter were sequenced (QG979998) and found 98% identical to CoRSV sequences available in GenBank. This is the first report of common bean as an experimental host of CoRSV. B. yothersi has been shown to transmit cileviruses. B. californicus seems to be the only vector of the dichorhavirus Orchid fleck virus, while B. phoenicis sensu stricto is the vector of the tentative dichorhavirus Citrus leprosis virus N. This is the first confirmation that B. papayensis can transmit CiLV-C and CoRSV. This is also the first time, following the reclassification of the B. phoenicis group, that a transmission was demonstrated by a single species of Brevipalpus for both a dichorha- and a cilevirus. Since this mite species is prevalent in coffee plantations in Brazil, it is likely the main vector of CoRSV under natural conditions.References:Beard, J. J., et al. 2015. Zootaxa 3944:1. https://doi.org/10.11646/zootaxa.3944.1.1 Crossref, ISI, Google ScholarKitajima, E. W., et al. 2011. Sci. Agric. 68:503. https://doi.org/10.1590/S0103-90162011000400017 Crossref, ISI, Google ScholarKubo, K., et al. 2011. Exp. Appl. Acarol. 54:33. https://doi.org/10.1007/s10493-011-9425-9 Crossref, ISI, Google ScholarLocali, E. C., et al. 2003. Plant Dis. 87:1317. https://doi.org/10.1094/PDIS.2003.87.11.1317 Link, ISI, Google ScholarFunding: Fapesp (2014/08458-9; 2015/23464-8) and CNPq (456824/2014-6).DetailsFiguresLiterature CitedRelated Vol. 102, No. 5 May 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Apr 2018Published: 8 Mar 2018First Look: 28 Nov 2017Accepted: 20 Nov 2017 Pages: 1046-1046 Information© 2018 The American Phytopathological SocietyCited byIntercropped Plants Provide a Reservoir of Predatory Mites in Coffee Crop24 January 2023 | Agriculture, Vol. 13, No. 2Infection by coffee ringspot virus is identified for the first time in succulent plants23 March 2022 | Journal of Plant Pathology, Vol. 104, No. 2A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae28 March 2022 | Frontiers in Microbiology, Vol. 13Citrus leprosis virus C (leprosis of citrus)CABI Compendium, Vol. CABI CompendiumBrevipalpus mites associated with coffee plants (Coffea arabica and C. canephora) in Chiapas, Mexico28 September 2021 | Experimental and Applied Acarology, Vol. 85, No. 1A Virus Infecting Hibiscus rosa-sinensis Represents an Evolutionary Link Between Cileviruses and Higreviruses3 May 2021 | Frontiers in Microbiology, Vol. 12Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas29 April 2021 | Frontiers in Microbiology, Vol. 12Survey of the citrus leprosis vector (Brevipalpus yothersi) and phytoseiids in spontaneous plants of an organic citrus orchard10 September 2020 | Experimental and Applied Acarology, Vol. 82, No. 2Brevipalpus Species Vectoring Citrus Leprosis Virus (Cilevirus and Dichorhavirus)25 April 2020 | Journal of Economic Entomology, Vol. 113, No. 4Brevipalpus-transmitted viruses: parallelism beyond a common vector or convergent evolution of distantly related pathogens?Current Opinion in Virology, Vol. 33Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants4 June 2018 | Archives of Virology, Vol. 163, No. 9Identification and Characterization of Citrus Chlorotic Spot Virus, a New Dichorhavirus Associated with Citrus Leprosis-Like SymptomsC. Chabi-Jesus, P. L. Ramos-González, A. D. Tassi, O. Guerra-Peraza, E. W. Kitajima, R. Harakava, J. E. A. Beserra, R. B. Salaroli, and J. Freitas-Astúa2 July 2018 | Plant Disease, Vol. 102, No. 8Dichorhaviruses in their Host Plants and Mite Vectors
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