Produção Nacional
Development of a rich fraction in phenolic compounds with high antioxidant and antimicrobial activity in Amburana cearensis seeds extracts
2017; Academic Journals; Volume: 11; Issue: 41 Linguagem: Inglês
10.5897/jmpr2017.6505
ISSN1996-0875
AutoresAires Aguiar Aline, Mendes Soares Ilsamar, Guerino Marson Poliana, Gerre Pereira Bastos Ernane, Donizeti Ascêncio Sérgio, Wagner de Souza Aguiar Raimundo,
Tópico(s)Essential Oils and Antimicrobial Activity
ResumoAmburana cearensis is a medicinal plant widely used in folk medicine. The purpose of this study was to identify the more appropriate extraction solvent for maximum antioxidant and antimicrobial effect. The extraction of A. cearensis seeds were carried out gradually to obtain the highest yields and constituents of the extracts using as solvent hexane, methanol, 80% alcohol and water in this sequence. Phytochemical screening showed phenolic compounds and thin layer chromatography (TLC) showed the flavonoid morin. Antioxidant activity was also evaluated by the method of scavenging the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH); rutin and ascorbic acid was used as standard; the hydro-alcoholic extract (EEA) showed an IC50 value of 17.95 μg/ml, similarly to the standards rutin and ascorbic acid, indicating high antioxidant action. The microdilution assay (MIC) showed antibacterial activity against the bacteria concentrations, values ≥0.25 mg/mL for EEA and aqueous (AEA) extracts against S. aureus, P. aeruginosa and S. flexneri. High performance liquid chromatography (HPLC) analysis of A. cearenses seed extracts revealed a high content of flavonoids and tannin compounds corroborating with TLC analysis. The extraction by exhaustion was very effective in exposing the bioactive principles of A. cearenses seeds showing their best compounds mainly in EEA extract. Key words: Amburana cearensis, exhaustion extraction, seed, phytochemical profile, antioxidant, antimicrobial activity.
Referência(s)